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Functional expression of the cDNA encoding for human lactate dehydrogenase-A in Chinese hamster ovary cells
1Laboratory of Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
Cell Biology International Reports
|October 1, 1987
Abstract:
The cloned cDNA encoding for human lactate dehydrogenase-A was inserted immediately downstream to the SV40 early promoter, and it was shown to synthesize the human LDH-A polypeptide in Chinese hamster ovary cells. The human LDH-A subunit and the endogenous Chinese hamster LDH-a subunit formed in vivo a heterotetrameric LDH-a3A1 functional isoenzyme, indicating the conserved tertiary structure of both LDH-A subunits.