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Updated: Nov 30, 2025

Fecal micro RNA Isolation

Fecal micro RNA Isolation

Published on: October 28, 2020

Fecal (micro) RNA Isolation.

Fyonn H Dhang¹, Howard L Weiner¹, Shirong Liu²

¹Ann Romney Center for Neurologic Diseases, Department of Neurology, Partners Multiple Sclerosis Center, Brigham and Women's Hospital and Harvard Medical School; Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women's Hospital.

Journal of Visualized Experiments : Jove

|November 16, 2020

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This study presents an optimized protocol for isolating high-purity RNA, including microRNAs, from animal and human fecal samples. The method yields sufficient RNA for downstream applications like RNA sequencing and RT-PCR.

Area of Science:

Gastroenterology
Molecular Biology
Biochemistry

Background:

RNA is present in animal and human gut lumen and feces.
Accurate isolation of fecal RNA is crucial for understanding gut microbiome and host-microbe interactions.
Existing methods may have limitations in purity, quantity, or sample processing capacity.

Purpose of the Study:

To describe an optimized protocol for isolating total RNA, including microRNAs, from fecal samples.
To achieve high purity and quantity of RNA suitable for downstream analyses.
To enhance existing RNA isolation techniques for fecal material.

Main Methods:

Isolation of total RNA and microRNAs from fecal samples.
Inclusion of additional washing steps for enhanced purity.

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Last Updated: Nov 30, 2025

Fecal micro RNA Isolation

Fecal micro RNA Isolation

Published on: October 28, 2020

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Published on: June 13, 2016

Murine Fecal Isolation and Microbiota Transplantation

Murine Fecal Isolation and Microbiota Transplantation

Published on: May 26, 2023

An improved resuspension method for increased RNA yield.

Specific tips for decontamination during the process.

Main Results:

The protocol effectively isolates highly purified RNA products.
Increased quantity of RNA is obtained compared to standard methods.
The method is suitable for downstream applications such as RNA sequencing, RT-PCR, and micro-array analysis.

Conclusions:

The optimized protocol provides high-purity, high-yield fecal RNA suitable for various molecular analyses.
Additional washing steps and improved resuspension contribute to the protocol's efficacy.
While samples >200 mg pose challenges, isolates from up to 200 mg are sufficient for most downstream applications.