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Compound K improves skin barrier function by increasing SPINK5 expression.

No-June Park1, Sim-Kyu Bong1, Sullim Lee2

  • 1Natural Products Research Institute, Korea Institute of Science and Technology, Gangneung, Republic of Korea.

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|November 16, 2020
PubMed
Summary

Compound K (CK) enhances skin barrier function by increasing SPINK5 expression, which helps regulate desquamation and protect against UV damage and atopic dermatitis. This suggests CK as a potential therapeutic for barrier-disrupted skin conditions.

Keywords:
Atopic dermatitisCompound KSPINK5Skin barrierUV

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Area of Science:

  • Dermatology
  • Biochemistry
  • Molecular Biology

Background:

  • The skin barrier protects against external threats.
  • Compound K (CK), a ginsenoside metabolite, is investigated for skin benefits.
  • SPINK5 (serine protease inhibitor Kazal type-5) is hypothesized to improve skin barrier function.

Purpose of the Study:

  • To investigate Compound K's effect on SPINK5 expression and skin barrier function.
  • To determine if CK can mitigate UVB-induced damage and atopic dermatitis-like conditions.

Main Methods:

  • Screening ginsenosides for SPINK5 promoter activity.
  • Assessing SPINK5, KLK5, KLK7, and PAR2 expression via RT-PCR and Western blotting in UVB-exposed HaCaT cells.
  • Evaluating transepidermal water loss (TEWL), hydration, and epidermal changes in UVB-irradiated mice and a DNCB-induced atopic dermatitis model.

Main Results:

  • CK increased SPINK5 expression and decreased downstream genes KLKs and PAR2.
  • CK treatment restored TEWL, hydration, and epidermal hyperplasia in UVB and DNCB models.
  • CK normalized SPINK5 expression, improving desquamation-related proteins.

Conclusions:

  • Compound K improves skin barrier function in models of UVB damage and atopic dermatitis via SPINK5.
  • CK shows potential as a therapeutic agent for diseases involving skin barrier disruption.