Circular RNA microarray expression profile and potential function of circ0005875 in clear cell renal cell carcinoma

Area of Science:

• Oncology
• Molecular Biology
• Genetics

Background:

• Circular RNAs (circRNAs) are novel noncoding RNAs implicated in various diseases, including cancers.
• Their role in clear cell renal cell carcinoma (ccRCC) tumorigenesis requires further investigation.

Purpose of the Study:

• To investigate the role of circRNAs in ccRCC development.
• To identify potential circRNA biomarkers for ccRCC.

Main Methods:

• Microarray analysis of circRNA expression profiles in ccRCC tissues and adjacent non-carcinoma tissues.
• Quantitative real-time PCR (qPCR) for validation of selected circRNAs.
• Functional assays (colony formation, Transwell, Matrigel-Transwell) to assess the role of hsa_circ_0005875 in ccRCC progression.
• Bioinformatic prediction and luciferase reporter assays to elucidate circRNA-miRNA interactions.

Main Results:

• Microarray analysis revealed 1988 significantly dysregulated circRNAs in ccRCC tissues.
• Hsa_circ_0005875 was significantly upregulated in ccRCC tissues and cells.
• Hsa_circ_0005875 expression correlated with tumor size, TNM stage, differentiation, and lymphatic metastasis.
• Overexpression of hsa_circ_0005875 enhanced ccRCC cell proliferation, migration, and invasion.
• Hsa_circ_0005875 acts as a competing endogenous RNA (ceRNA) for hsa_miR-145-5p, potentially upregulating ZEB2.

Conclusions:

• Hsa_circ_0005875 plays a role in promoting ccRCC tumor growth and metastasis.
• Hsa_circ_0005875 is a potential diagnostic and prognostic biomarker for ccRCC.