Genome-wide study of flowering-related MADS-box genes family in Cardamine hirsuta

MADS-box genes take part in diverse biological functions especially in development of reproductive structures and control of flowering time. Recently, Cardamine hirsuta has emerged as an exclusively powerful genetic system in comparative studies of development. Although the C. hirsuta genome sequence is available but a comprehensive analysis of its MADS-box family genes is still lacking. Here, we determined 50 Cardamine MADS-box genes through bioinformatics tools and classified them into 2 Mβ, 6 Mα and 2 Mγ and 40 MIKC-type (35 MIKCc and 5MIKC*) genes based on a phylogenetic analysis. The C. hirsuta MIKC subfamily could be further classified into 14 subgroups as Arabidopsis. However the number of MADS-box proteins was not equal among these subgroups. Based on the structural diversity among 50 MADS-box genes, 2 lineages were obtained, type I and type II. The lowest number of introns (0 or 1) was found in the Mα, Mβ, and Mγ groups of the type I genes. The most Cardamine MADS-box genes were randomly distributed on only three chromosomes. C. hirsuta had a relatively lower number of flowering MADS-box genes than A. thaliana and probably tandem duplication event resulted in the expansion of FLC, SQUA and TM3 family members in Arabidopsis. Moreover among the conserved motifs, ChMADS5 of SQUA, ChMADS34 of TM3 and ChMADS51 of AGL15 families had no K-domain. This study provides a basis for further functional investigation of MADS-box genes in C. hirsuta.