Related Experiment Video

Updated: Nov 30, 2025

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

18.3K

Corrigendum: Directed Evolution of Pseudomonas fluorescens Lipase Variants With Improved Thermostability Using

Lijun Guan1, Yang Gao1, Jialei Li1

  • 1Institute of Food Processing, Heilongjiang Academy of Agricultural Sciences, Harbin, China.

Frontiers in Bioengineering and Biotechnology
|November 16, 2020
PubMed

Abstract:

[This corrects the article DOI: 10.3389/fbioe.2020.01034.].

Keywords:
lipasemethanol tolerancesite-directed mutagenesisstructural analysisthermostability

More Related Videos

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

7.6K
Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
09:01

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Published on: March 16, 2011

30.9K

Related Experiment Videos

Last Updated: Nov 30, 2025

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

18.3K
In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

7.6K
Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
09:01

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Published on: March 16, 2011

30.9K

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.4K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.4K

Articles linked to this work by shared authors, journal, and citation graph.

Early recognition and bundle-based management of pediatric sepsis in the emergency department: a narrative review.

Translational pediatrics·2026

Expansion and functional diversification of terpene synthases shape volatile terpenoid landscape in Cannabis sativa.

Acta pharmaceutica Sinica. B·2026

Cystic Fibrosis: Modern Diagnostic and Therapeutic Advances from Molecular Pathogenesis to Multidisciplinary Management.

Genes·2026

Modulating N2O emission in partial denitrification-anammox system: The critical role of carbon source-driven shifts in carbon-nitrogen metabolic balance.

Environmental research·2026

All-trans retinoic acid suppresses systemic inflammation induced by severe fever with thrombocytopenia syndrome virus in mice.

Nature microbiology·2026

Metagenomic and Metabolomic Insights into Volatile Flavor Changes and Microbial Community Shifts in Physalis pubescens L. Fermentation by Lactiplantibacillus plantarum.

Molecules (Basel, Switzerland)·2026

From pathogenesis to therapy: extracellular vesicles in osteoarthritis.

Frontiers in bioengineering and biotechnology·2026

Cu-CeO2 NP-loaded HAMA hydrogel patches for promoting high-quality cutaneous wound healing.

Frontiers in bioengineering and biotechnology·2026

Construction of novel phycocyanin conjugated chitosan-TiO2-Fe3O4 nanoparticles for Lactobacillus retainment and application in cyclic batch fermentation.

Frontiers in bioengineering and biotechnology·2026

3D foot and ankle radiographic measurement toolbox.

Frontiers in bioengineering and biotechnology·2026

Generation of alveolar organoids from human induced pluripotent stem cells using a stirred-tank bioreactor.

Frontiers in bioengineering and biotechnology·2026

Magnetic particle clusters with tunable thrombus penetration for enhanced thrombolysis.

Frontiers in bioengineering and biotechnology·2026

Spin Engineering of Ni-Based High-Entropy Oxide Cocatalyst: Synchronously Optimizing Intermediate Adsorption and Charge Carrier Separation for Photocatalytic Hydrogen Evolution.

Advanced materials (Deerfield Beach, Fla.)·2026

Green electrochemical determination of capsaicin using a sensor constructed from cattle manure and spent battery graphite.

Analytical and bioanalytical chemistry·2026

De-novo transcriptome sequencing and annotation of midgut gland tissue from edible crab (Cancer pagurus) and clawed lobsters (Homarus americanus, H. gammarus).

Marine genomics·2026

Orbital-tailoring strategy via defect engineering in NiCo2S4/CdSe heterojunction for enhanced photocatalytic hydrogen evolution.

Journal of colloid and interface science·2026

Y-type molecular sieve framework for efficient enrichment of urinary serotonin prior to screening for type 2 diabetes by LC-MS/MS.

Mikrochimica acta·2026

Multi-Subunit Engineering of Photorespiratory Glycine Decarboxylase Refines Photosynthesis, Growth and Metabolic Robustness of Arabidopsis.

Plant, cell & environment·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us