Short DNA Oligonucleotide as a Ag+ Binding Detector
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Updated: Nov 30, 2025

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
Published on: February 9, 2024
Fusheng Shen1,2, Song Mao1,2, Johnsi Mathivanan1,2
1Department of Chemistry, University at Albany, State University of New York, 1400 Washington Ave., Albany, New York 12222, United States.
Abstract:
Ag+ has been known to mediate several natural metallo-base pairs. Based on the unique structural information of a short 8-mer DNA strand (5'-GCACGCGC-3') induced by Ag+, we constructed several fluorescent DNA beacons for the detection of Ag+ according to the increase in the fluorescence emission on Ag+ binding. This Ag+ detection assay is quick, sensitive, and easy to adapt and can function in a wide range of temperatures from 5 to 65 °C.
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