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Control of adipogenic commitment by a STAT3-VSTM2A axis.

Manal Al Dow1,2, Maruhen Amir Datsch Silveira2,3, Audrée Poliquin1,2

  • 1Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec (CRIUCPQ), Faculté de Médecine, Université Laval, Québec, Canada.

American Journal of Physiology. Endocrinology and Metabolism
|November 16, 2020
PubMed
Summary
This summary is machine-generated.

Signal transducer and activator of transcription 3 (STAT3) controls the expression of V-set and transmembrane domain-containing protein 2A (VSTM2A) in white adipose tissue. STAT3 activation is linked to early white adipose tissue development and VSTM2A expression.

Keywords:
STAT3VSTM2Aadipogenesisadipose tissuepreadipocytes

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Area of Science:

  • Cell Biology
  • Metabolism
  • Molecular Biology

Background:

  • White adipose tissue (WAT) is vital for metabolic homeostasis, with adipocyte differentiation crucial for lipid storage.
  • Adipose progenitor identity and recruitment signals remain incompletely understood.
  • V-set and transmembrane domain-containing protein 2A (VSTM2A) is a novel protein promoting adipogenesis.

Purpose of the Study:

  • To elucidate the molecular mechanisms regulating VSTM2A expression in preadipocytes.
  • To identify key signaling pathways and transcription factors involved in VSTM2A regulation.

Main Methods:

  • Treatment of preadipocytes with various compounds modulating adipogenesis regulators.
  • Analysis of VSTM2A expression in response to pathway activation/inhibition.
  • Identification and validation of transcription factors affecting VSTM2A expression.
  • In vivo studies in mice to assess STAT3 phosphorylation during WAT development.

Main Results:

  • VSTM2A expression is positively regulated by PI3K/mTOR and cAMP signaling pathways.
  • MAPK pathway and glucocorticoid receptor repress VSTM2A expression.
  • Signal transducer and activator of transcription 3 (STAT3) was identified as a key transcription factor upregulating VSTM2A.
  • STAT3 activation increased VSTM2A expression, while inhibition decreased it.
  • STAT3 phosphorylation is elevated during early WAT development, correlating with VSTM2A expression.

Conclusions:

  • STAT3 is a critical transcription factor governing VSTM2A expression in preadipocytes.
  • The findings provide insights into the regulation of adipogenesis and VSTM2A function.
  • STAT3 activation during early WAT development highlights its role in adipose tissue expansion.