Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

11.2K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.2K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

20.0K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
20.0K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.2K
3.2K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

9.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.5K
Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

4.5K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
4.5K
Alternative RNA Splicing02:18

Alternative RNA Splicing

24.0K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
24.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identifying Potential Exosome-Derived mRNA Biomarkers for Diagnosis and Prediction of Breast Cancer Using Machine-Learning Approaches.

Interdisciplinary sciences, computational life sciences·2026
Same author

Diagnostic Value of Bilateral Pectoralis Major Total Cross-Sectional Area in Patients with AECOPD Complicated by Malnutrition.

International journal of chronic obstructive pulmonary disease·2026
Same author

Metabolomic and phytochemical insights into tree age effects on <i>Lycium barbarum</i> fruits quality.

Food chemistry: X·2026
Same author

KRAS<sup>G12V/A146T</sup> mutations are associated with nCRT resistance via enhanced DNA double-strand break repair and support a deep learning prediction framework in LARC.

Cellular & molecular biology letters·2026
Same author

Hepatoprotective effect of <i>Olea europaea</i> L. leaf against oxidative stress in hepatocytes and zebrafish embryos.

Natural product research·2026
Same author

ATP-responsive NIR-â…¡ fluorescence/mass spectrometry dual-modal nanoprobe for precision diagnosis of metabolic dysfunction-associated steatohepatitis.

Analytica chimica acta·2026

Related Experiment Video

Updated: Nov 30, 2025

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
06:34

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Published on: January 21, 2020

8.7K

NONCODEV6: an updated database dedicated to long non-coding RNA annotation in both animals and plants.

Lianhe Zhao1,2, Jiajia Wang3,4, Yanyan Li3,4

  • 1Key Laboratory of Intelligent Information Processing, Advanced Computer Research Center, Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100190, China.

Nucleic Acids Research
|November 16, 2020
PubMed
Summary

The NONCODEv6 database now includes over 644,000 long non-coding RNAs (lncRNAs) for animals and plants. This update features enhanced human lncRNA-cancer links and new plant lncRNA data.

More Related Videos

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

2.8K
Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
10:40

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

10.8K

Related Experiment Videos

Last Updated: Nov 30, 2025

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
06:34

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Published on: January 21, 2020

8.7K
mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

2.8K
Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
10:40

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

10.8K

Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Noncoding RNAs (ncRNAs), particularly long non-coding RNAs (lncRNAs), play crucial roles in biological processes.
  • Databases are essential for organizing and accessing the rapidly growing volume of ncRNA data.
  • Previous versions of NONCODE have provided valuable resources for lncRNA research.

Purpose of the Study:

  • To present NONCODEv6, an updated and expanded version of the comprehensive noncoding RNA database.
  • To incorporate a broader scope of lncRNAs, including those from plants, alongside animals.
  • To introduce new features enhancing the utility of the database for researchers.

Main Methods:

  • Collection and curation of lncRNA data from various species.
  • Annotation of lncRNAs with functional and expression information.
  • Integration of transcriptome sequencing data to update lncRNA-disease relationships, particularly for cancer.
  • Transcript-level conservation analysis for plant species.

Main Results:

  • NONCODEv6 contains 644,510 lncRNAs, an increase from 548,640 in the previous version.
  • Specific increases noted in human (173,112) and mouse (131,974) lncRNAs.
  • Introduction of 94,697 plant lncRNAs.
  • Updated human lncRNA-cancer associations based on new data.
  • New annotations for plant lncRNAs, including tissue expression, predicted functions, and conservation.

Conclusions:

  • NONCODEv6 represents a significant expansion of the NONCODE database, covering both animals and plants.
  • The updated features, especially concerning human lncRNA-cancer relationships and plant lncRNA data, enhance its value for biological research.
  • The database provides a crucial resource for exploring the functions and evolution of lncRNAs.