Sex differences in the autonomic and cerebrovascular responses to upright tilt

Sex differences in the regulation of autonomic and cerebrovascular responses to orthostatic stress remain unclear. The objectives of this study were to concurrently investigate autonomic control and cerebrovascular resistance indices, including critical closing pressure (CrCP) and resistance area product (RAP), during upright tilt in men and women. In 13 women and 14 men (18-29 years), ECG, non-invasive blood pressure, middle cerebral artery blood velocity, and end-tidal CO₂ (ETCO₂) were continuously measured during supine rest and 70° tilt. Heart rate variability (HRV), cardiovagal baroreflex sensitivity (cBRS), and transfer function parameters of dynamic cerebral autoregulation were calculated. Compared to supine, upright tilt increased the low frequency-to-high frequency ratio of HRV in men only (P = 0.044), and decreased cBRS more in women (P = 0.001). Cerebrovascular resistance index (CVRi) increased during tilt only in men (sex-by-time interaction: P = 0.004). RAP was lower in women throughout tilt (main effect of sex: P = 0.022). CrCP decreased during tilt in both sexes (main effect of time: P < 0.001). Normalizing to ETCO2 did not alter the effect of tilt on cerebrovascular resistance. Men displayed a greater increase of sympathetic indices and CVRi during tilt while women had greater parasympathetic withdrawal. We hypothesize that increased sympathetic activity in men may drive sex differences in the cerebrovascular response to upright posture.