Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The electroanalytical approach to lipid peroxide determinations.

M O Funk1

  • 1Department of Chemistry, University of Toledo, OH 43606.

Free Radical Biology & Medicine
|January 1, 1987
PubMed
Summary

This study introduces a sensitive electroanalytical method using electrochemical detection with high-performance liquid chromatography (HPLC) to measure lipid peroxides in biological samples. The technique achieves detection limits in the low nanogram range for fatty acid hydroperoxides.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Three-dimensional structure of a purple lipoxygenase.

Journal of the American Chemical Society·2001
Same author

Iron extraction from soybean lipoxygenase 3 and reconstitution of catalytic activity from the apoenzyme.

Biochemical and biophysical research communications·2001
Same author

Structural and thermochemical characterization of lipoxygenase-catechol complexes.

Biochemistry·1999
Same author

Structure of soybean lipoxygenase L3 and a comparison with its L1 isoenzyme.

Proteins·1997
Same author

Flash-freezing causes a stress-induced modulation in a crystal structure of soybean lipoxygenase l3.

Acta crystallographica. Section D, Biological crystallography·1996
Same author

Catalysis sensitive conformational changes in soybean lipoxygenase revealed by limited proteolysis and monoclonal antibody experiments.

Biochemistry·1995

Area of Science:

  • Analytical Chemistry
  • Electrochemistry
  • Biochemistry

Background:

  • Lipid peroxides are crucial biomarkers in physiological material.
  • Accurate determination of lipid peroxides is essential for understanding oxidative stress.
  • Existing methods may lack sufficient sensitivity for certain applications.

Purpose of the Study:

  • To develop a highly sensitive electroanalytical method for lipid peroxide determination.
  • To utilize electrochemical detection coupled with HPLC for enhanced sensitivity.
  • To establish a reliable method for analyzing lipid peroxides in physiological samples.

Main Methods:

  • Employed an electroanalytical technique with electrochemical detection.
  • Utilized high-performance liquid chromatography (HPLC) with reversed-phase separation.

Related Experiment Videos

  • Adapted a modified polarographic detector for direct sample analysis.

    • Main Results:

    • Achieved high sensitivity for the determination of lipid peroxides.
    • Established low nanogram detection limits for fatty acid hydroperoxides.
    • Demonstrated the applicability of the method for analyzing organic peroxides in physiological material.

    Conclusions:

    • The described electroanalytical method offers enhanced sensitivity for lipid peroxide analysis.
    • This technique provides a valuable tool for the quantification of lipid peroxides in biological contexts.
    • The method is suitable for direct analysis of samples containing organic peroxides.