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Insulin/Glucose-Responsive Cells Derived from Induced Pluripotent Stem Cells: Disease Modeling and Treatment of

Sevda Gheibi1, Tania Singh1, Joao Paulo M C M da Cunha1

  • 1Unit of Molecular Metabolism, Lund University Diabetes Centre, Jan Waldenströms gata 35, Box 50332, SE-202 13 Malmö, Sweden.

Cells
|November 17, 2020
PubMed

Insights

Restoring insulin-producing and insulin-responsive cells from stem cells offers a promising strategy for type 2 diabetes treatment. While challenges remain, induced pluripotent stem cells show potential for generating crucial cell types for therapy and disease modeling.

Area of Science:

  • Stem cell biology
  • Endocrinology
  • Regenerative medicine

Background:

  • Type 2 diabetes involves pancreatic beta-cell dysfunction and insulin resistance, lacking ideal comprehensive therapies.
  • Restoring functional insulin-producing and insulin-responsive cells is a logical therapeutic approach.
  • Stem cell-derived cell generation in vitro is a key area for diabetes research.

Purpose of the Study:

  • To review the generation of induced pluripotent stem cells (iPSCs).
  • To discuss the differentiation of iPSCs into pancreatic beta-like, myotube, adipocyte-like, and hepatocyte-like cells.
  • To explore the potential of these cells for disease modeling and replacement therapies.

Main Methods:

  • Review of current literature on induced pluripotent stem cell generation.
  • Description of differentiation protocols for iPSCs into various cell types.
  • Discussion of challenges and future directions in the field.

Main Results:

  • Pluripotent stem cells, including iPSCs, are viable sources for insulin-secreting and glucose-responsive cells.
  • Differentiation protocols can generate pancreatic beta-like cells, myotubes, adipocyte-like, and hepatocyte-like cells from iPSCs.
  • Utilizing these cells for human disease modeling is feasible.

Conclusions:

  • Generating robust, glucose/insulin-responsive cells from stem cells remains a significant challenge.
  • Stem cell-derived cells hold promise for type 2 diabetes replacement therapies.
  • Further research is essential to overcome current limitations and advance therapeutic development.
Keywords:
adipocytediabeteshepatocyteiPSCinsulin resistancemyotubeβ-cells

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