Setting the Stage: Genes Controlling Mechanosensation and Ca²⁺ Signaling in Escherichia coli

Although mechanistic understanding of calcium signaling in bacteria remains inchoate, current evidence clearly links Ca²⁺ signaling with membrane potential and mechanosensation. Adopting a radically new approach, Luder et al. scanned the Keio collection of Escherichia coli gene knockouts (R. Luder, G. N. Bruni, and J. M. Kralj, J Bacteriol 203:e00509-20, 2021, https://doi.org/10.1128/JB.00509-20) to identify mutations that cause changes in Ca²⁺ transients. They identify genes associating Ca²⁺ signaling with outer membrane biogenesis, proton motive force, and, surprisingly, long-term DNA damage. Their work has major implications for electrophysiological communication between bacteria and their environment.