Related Experiment Video

Updated: Nov 30, 2025

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

15.9K

Potential Mobilization of mcr-10 by an Integrative Mobile Element via Site-Specific Recombination in Cronobacter

Jing Yang1,2,3, Lina Liu4, Yu Feng1,3,4

  • 1Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China.

Antimicrobial Agents and Chemotherapy
|November 17, 2020
PubMed

Insights

The newly discovered mcr-10 colistin resistance gene

Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • The mcr-10 gene confers resistance to colistin, a crucial antibiotic.
  • The mechanism of mcr-10 gene spread is not well understood.
  • Cronobacter sakazakii is an opportunistic pathogen.

Purpose of the Study:

  • To investigate the mobilization mechanism of the mcr-10 gene.
  • To identify the genetic context of mcr-10 in a Cronobacter sakazakii isolate.

Main Methods:

  • Plasmid analysis
  • Comparative genomics
  • Identification of recombination sites

Main Results:

  • The mcr-10 gene was located on an IncFIB plasmid in Cronobacter sakazakii.
  • This plasmid also carried virulence genes: mrkABCDFJ, iucABCD/iutA, and eitCBAD.
  • Two recombination sites flanking the mcr-10 genetic element were identified.

Conclusions:

  • Site-specific recombination mediated by an integrase is a likely mechanism for mcr-10 mobilization.
  • Understanding mcr-10 mobilization is critical for controlling antibiotic resistance spread.
Keywords:
Cronobacter sakazakiicolistincolistin resistanceintegrasemcrmcr-10site-specific recombination

More Related Videos

Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria09:38

Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria

Published on: February 2, 2021

4.2K
Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus09:03

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

12.2K

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.4K
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.2K
Mechanism of Conjugation01:19

Mechanism of Conjugation

Bacterial conjugation is a mechanism of horizontal gene transfer that enables the exchange of genetic material between bacterial cells through direct contact. This process is facilitated by a donor cell carrying a conjugative plasmid, which encodes genes necessary for pilus formation, DNA replication, and transfer. The conjugative plasmid plays a central role in initiating and executing the transfer of genetic material.The tra region of the conjugative plasmid encodes proteins responsible for...
468
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
55.5K
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
445