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Chemical Shift: Internal References and Solvent Effects

Chemical Shift: Internal References and Solvent Effects

In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...

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Related Experiment Video

Updated: Nov 30, 2025

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

RefMet: a reference nomenclature for metabolomics

Eoin Fahy¹, Shankar Subramaniam²

¹Department of Bioengineering, University of California San Diego, La Jolla, CA, USA. efahy@ucsd.edu.

|November 17, 2020

Summary

No abstract available in PubMed .

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