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Distribution and cellular localization of actin depolymerizing factor.

J R Bamburg1, D Bray

  • 1Medical Research Council Cell Biophysics Unit, Kings College, London, England.

The Journal of Cell Biology
|December 1, 1987
PubMed
Summary
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Actin depolymerizing factor (ADF) is abundant in most vertebrate tissues, regulating actin filaments. Its levels vary during development and are low in adult muscle, suggesting specific cellular roles.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Developmental Biology

Background:

  • Actin depolymerizing factor (ADF) is a 19 kD protein that complexes with actin.
  • ADF plays a role in regulating actin dynamics within cells.

Purpose of the Study:

  • To analyze the distribution and cellular localization of ADF in various vertebrate tissues.
  • To investigate the developmental changes in ADF levels, particularly in skeletal muscle.

Main Methods:

  • Raised a rabbit antiserum against chick brain ADF.
  • Utilized the antiserum for Western blot analysis and indirect immunofluorescence.
  • Examined ADF presence in embryonic and adult chick tissues, cultured cell lines, and invertebrate extracts.

Main Results:

Related Experiment Videos

  • ADF is a major protein (0.1-0.4%) in most embryonic and adult chick tissues, with high molar ratios to actin in some.
  • Adult heart and skeletal muscle exhibit very low ADF levels (<0.02%).
  • ADF levels peak in developing skeletal muscle and are conserved across vertebrate species but absent in tested invertebrates.

Conclusions:

  • ADF is widely distributed in vertebrate cells, found in the cytosol and at dynamic structures like growth cones.
  • Its abundance and actin-sequestering ability indicate a significant role in regulating actin filaments.
  • Differential expression suggests tissue-specific functions and developmental regulation of ADF.