Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cytophysiological studies on isolated pancreatic islets in vitro.

B G Slavin, P M Beigelman, S P Bessman

    The Anatomical Record
    |August 1, 1977
    PubMed
    Summary

    Isolated pancreatic islets showed decreased structural integrity over time, with higher glucose levels generally increasing insulin release. Beta-cell activity, including insulin synthesis and secretion, was observed during incubation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Adipose tissue deficiency, glucose intolerance, and increased atherosclerosis result from mutation in the mouse fatty liver dystrophy (fld) gene.

    Journal of lipid research·2000
    Same author

    The fatty liver dystrophy mutant mouse: microvesicular steatosis associated with altered expression levels of peroxisome proliferator-regulated proteins.

    Journal of lipid research·1998
    Same author

    Historical perspective: tyrosine and maternal phenylketonuria, welcome news.

    The American journal of clinical nutrition·1998
    Same author

    Insulin as a probe of mitochondrial metabolism in situ.

    Molecular and cellular biochemistry·1997
    Same author

    Hexokinase binding to mitochondria: a basis for proliferative energy metabolism.

    Journal of bioenergetics and biomembranes·1997
    Same author

    Impaired mitochondrial metabolism and reduced amphibolic Krebs cycle activity in diabetic rat hepatocytes.

    Biochemistry and molecular biology international·1995

    Area of Science:

    • Endocrinology
    • Cell Biology
    • Histology

    Background:

    • Pancreatic islets are crucial for glucose homeostasis.
    • Understanding beta-cell function in response to glucose is vital for diabetes research.

    Purpose of the Study:

    • To investigate the structural and functional changes in isolated pancreatic islets under varying glucose concentrations and incubation times.
    • To correlate cytologic observations with insulin release and synthesis.

    Main Methods:

    • Incubation of isolated pancreatic islets from mice and rats.
    • Exposure to high (300 mg%) and low (50 mg%) glucose levels for 0.5-60 minutes.
    • Light and electron microscopy for cytologic examination.
    • Measurement of insulin release.

    Related Experiment Videos

    Main Results:

    • Islet structural integrity decreased progressively with incubation time, irrespective of glucose levels.
    • High glucose generally enhanced insulin release compared to low glucose, except at 30 seconds.
    • Cytologic changes in beta-cells, including degranulation and signs of insulin synthesis, correlated with insulin secretion peaks.

    Conclusions:

    • Incubation affects islet structure and function, with time and glucose concentration being key factors.
    • Cytologic examination provides valuable insights into the physiological and biochemical responses of pancreatic islets.
    • Correlating structural changes with functional output is essential for a comprehensive understanding of beta-cell activity.