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A Multifunctional Electrochemical Sensor for the Simultaneous Detection of Ascorbic Acid, Dopamine, Uric Acid, and
Huimin Wang1, Xueli Zhang1, Shuangjue Wang1
1State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, P.R. China.
Background:
Ascorbic acid (AA), dopamine (DA), uric acid (UA), and nitrite (NO2-) are essential biomarkers for human metabolism and can be used to indicate some chronic diseases and metabolic disorders, including scurvy, Parkinson's disease, hyperuricemia, and kidney disease.
Objective:
A multifunctional electrochemical sensor that can integrate the detection of these species was constructed using nanoporous gold (NPG) as a recognition element to modify glassy carbon electrode (GCE).
Methods:
The electrochemical performance of the multifunctional electrochemical sensor was investigated toward AA, DA, UA, and NO2- in citrate buffer solution (CBS, 100 mM, pH 4.0) and human serum using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) methods.
Results:
In the quaternary mixture detection, the resulting NPG/GCE electrode displayed four independent oxidation peaks with wide peak separations. Further, the NPG/GCE electrode showed good linear responses with the sensitivities of 32, 1103, 71, and 147 μA/mM/cm2 and the detection limits of 1.58, 0.17, 0.37, and 0.36 μM for AA, DA UA, and NO2-, respectively. Additionally, the NPG/GCE electrode exhibited great anti-interference and was successfully applied in human serum samples.
Conclusions:
These results indicate that the NPG/GCE electrode can simultaneously and selectively detect AA, DA, UA, and NO2-, which has the potential for application and diagnosis in the screening and diagnosis of chronic diseases and metabolic disorders.
Highlights:
A multianalyte electrochemical sensor was fabricated for human metabolites detection. The sensor displayed good performance in the simultaneous detection of AA, DA, UA, and NO2- and applied to human serum samples.
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