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Deciphering the Dynamic Landscape of Transcription-Associated mRNP Quality Control Components Over the Whole Yeast

Kévin Moreau1, Aurélia Le Dantec1, A Rachid Rahmouni2

  • 1Centre de Biophysique Moléculaire, UPR 4301 du CNRS, Orléans, France.

Methods in Molecular Biology (Clifton, N.J.)
|November 17, 2020
PubMed

Insights

Eukaryotic cells use a surveillance system to degrade faulty messenger RNAs (mRNAs). This system, involving the NNS complex and TRAMP, coordinates with transcription to ensure mRNA quality control.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Eukaryotic cells possess a quality control system to identify and degrade aberrant messenger ribonucleoprotein complexes (mRNPs) with processing and packaging defects.
  • This surveillance mechanism, involving the NNS complex (Nrd1-Nab3-Sen1) and TRAMP complex (Trf4-Air2-Mtr4), targets defective mRNAs for degradation by the exosome-associated exonuclease Rrp6.
  • The NNS complex also plays a role in the termination, processing, and decay of non-coding RNAs (ncRNAs).

Purpose of the Study:

  • To visualize the dynamic movement and coordination of mRNP quality control components across yeast chromosomes.
  • To understand how the surveillance system physically and functionally interacts with the transcription machinery during perturbations of mRNP biogenesis.
  • To investigate the impact of mRNP quality control on ncRNA processing and termination.

Main Methods:

  • Developed a genome-wide approach to visualize the dynamic movement of quality control factors.
  • Perturbed mRNP biogenesis to study the surveillance system's response.
  • Monitored the recruitment of NNS and TRAMP complexes to mRNA and ncRNA genes.

Main Results:

  • Demonstrated the dynamic recruitment of quality control components to specific genomic locations.
  • Showed precise coordination between the surveillance system and transcription machinery in detecting faulty mRNA events.
  • Observed that recruitment of quality control factors to defective mRNA genes occurs at the expense of their association with ncRNA genes, leading to ncRNA processing defects.

Conclusions:

  • The mRNP quality control system is dynamically coordinated with transcription to ensure mRNA integrity.
  • The recruitment of surveillance factors to aberrant mRNAs can disrupt the processing and termination of ncRNAs.
  • This study provides insights into the intricate balance between mRNA quality control and ncRNA metabolism.
Keywords:
ChIP-seqRNA-seqYeastmRNP biogenesismRNP quality controlncRNAs

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