Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Introduction to Fibroblasts01:09

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitochondrial membrane hyperpolarization modulates nuclear DNA methylation and gene expression through phospholipid remodeling.

Nature communications·2025
Same author

Mitochondrial membrane potential regulates nuclear DNA methylation and gene expression through phospholipid remodeling.

bioRxiv : the preprint server for biology·2024
Same author

Author Correction: Polλ promotes microhomology-mediated end-joining.

Nature structural & molecular biology·2023
Same author

Temporal recruitment of base excision DNA repair factors in living cells in response to different micro-irradiation DNA damage protocols.

DNA repair·2023
Same author

Polλ promotes microhomology-mediated end-joining.

Nature structural & molecular biology·2022
Same author

A common transcriptional mechanism involving R-loop and RNA abasic site regulates an enhancer RNA of APOE.

Nucleic acids research·2022
Same journal

Sperm-Binding Assay Using an In Vitro 3D Model of the Mammalian Cumulus-Oocyte Complex.

Current protocols in toxicology·2020
Same journal

An Open-Globe Porcine Injury Platform for Assessing Therapeutics and Characterizing Biological Effects.

Current protocols in toxicology·2020
Same journal

A Protocol to Study Mitochondrial Function in Human Neural Progenitors and iPSC-Derived Astrocytes.

Current protocols in toxicology·2020
Same journal

Measuring Changes in Keap1-Nrf2 Protein Complex Conformation in Individual Cells by FLIM-FRET.

Current protocols in toxicology·2020
Same journal

In Vitro Evaluation of Toxicant Influences on the Immune System.

Current protocols in toxicology·2020
Same journal

Analysis of Busulfan in Plasma by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).

Current protocols in toxicology·2020
See all related articles

Related Experiment Video

Updated: Jun 29, 2026

The Use of Primary Human Fibroblasts for Monitoring Mitochondrial Phenotypes in the Field of Parkinson's Disease
15:09

The Use of Primary Human Fibroblasts for Monitoring Mitochondrial Phenotypes in the Field of Parkinson's Disease

Published on: October 3, 2012

17.1K

Using Human Primary Foreskin Fibroblasts to Study Cellular Damage and Mitochondrial Dysfunction.

Cristina A Nadalutti1, Samuel H Wilson1

  • 1Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, Raleigh, North Carolina.

Current Protocols in Toxicology
|November 17, 2020
PubMed
Summary
This summary is machine-generated.

This study presents three simple protocols for assessing human primary foreskin fibroblast (FSK) cell viability and mitochondrial function. These methods utilize readily available materials for accurate in vitro research, enhancing drug development models.

Keywords:
cellular damagefibroblastsmitochondriamitophagytransmission electron microscopy

More Related Videos

Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin
10:36

Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin

Published on: March 23, 2022

7.0K
Tissue Processing and Isolation of Primary Fibroblasts from the Human Vagina
08:30

Tissue Processing and Isolation of Primary Fibroblasts from the Human Vagina

Published on: November 22, 2024

789

Related Experiment Videos

Last Updated: Jun 29, 2026

The Use of Primary Human Fibroblasts for Monitoring Mitochondrial Phenotypes in the Field of Parkinson's Disease
15:09

The Use of Primary Human Fibroblasts for Monitoring Mitochondrial Phenotypes in the Field of Parkinson's Disease

Published on: October 3, 2012

17.1K
Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin
10:36

Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin

Published on: March 23, 2022

7.0K
Tissue Processing and Isolation of Primary Fibroblasts from the Human Vagina
08:30

Tissue Processing and Isolation of Primary Fibroblasts from the Human Vagina

Published on: November 22, 2024

789

Area of Science:

  • Cell Biology
  • Mitochondrial Research
  • Biotechnology

Background:

  • Cell lines are common research models but may not reflect in vivo conditions.
  • Human primary cells offer more relevant data due to their preserved in vivo characteristics.
  • Human foreskin fibroblasts (FSK) are valuable primary cells isolated from discarded surgical tissue.

Purpose of the Study:

  • To describe three straightforward protocols for studying cellular viability and mitochondrial function in FSK cells.
  • To provide researchers with accessible methods for analyzing primary human cells.
  • To establish reliable techniques for evaluating mitochondrial health and cellular damage.

Main Methods:

  • Isolation and culture of human primary foreskin fibroblasts (FSK) from clinical tissue.
  • Determination of cell viability using crystal violet staining.
  • Assessment of mitochondrial status and cellular damage via transmission electron microscopy.

Main Results:

  • Successfully isolated and maintained FSK cells.
  • Established protocols for reliable cell viability and mitochondrial function assessment.
  • Demonstrated the utility of these methods in recapitulating physiological conditions and studying toxic effects.

Conclusions:

  • The presented protocols offer easy and effective methods for studying FSK cells.
  • These techniques enhance the study of cellular health, metabolism, and disease models.
  • The methods provide valuable tools for research in drug development and human health.