Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Analgesia and Pain Management01:25

Analgesia and Pain Management

1.2K
Pain is critical to various clinical pathologies, provoking an urgent need for effective management. Pain, whether acute or chronic, is a complex neurochemical process. Its alleviation depends on the type, with nonopioid analgesics effective for mild to moderate pain, such as musculoskeletal or inflammatory pain, while neuropathic pain responds best to anticonvulsants, tricyclic antidepressants, or serotonin/norepinephrine reuptake inhibitors. For severe acute or chronic pain, opioids may be...
1.2K
Acute Kidney Injury II: Pathophysiology01:29

Acute Kidney Injury II: Pathophysiology

588
Acute kidney injury (AKI) causes are categorized into three primary categories based on the location of the injury: prerenal, intrarenal (or intrinsic), and postrenal causes. This classification guides clinical management and illustrates how different pathways can impair kidney function.Etiology and Pathophysiology of Acute Kidney Injury1. Prerenal causesEtiology: Prerenal Acute Kidney Injury, the most common type, occurs when reduced blood flow to the kidneys decreases filtration capacity...
588
Acute Kidney Injury IV: Diagnostic Studies and Prevention01:30

Acute Kidney Injury IV: Diagnostic Studies and Prevention

159
Accurate diagnosis and effective prevention are critical in managing Acute Kidney Injury (AKI), which is linked to high mortality rates ranging from 10% to 80%. Timely recognition of at-risk patients and careful monitoring can significantly reduce the likelihood of kidney damage.Diagnostic Assessments:The diagnostic process starts with a comprehensive medical history to identify prerenal, intrarenal, and postrenal causes.Prerenal causes, such as dehydration, hypotension, or blood loss, should...
159
Heart Failure Drugs: Inhibitors of Renin-Angiotensin System01:26

Heart Failure Drugs: Inhibitors of Renin-Angiotensin System

690
The activation of the sympathetic nervous system and the renin-angiotensin-aldosterone system (RAAS) contributes to cardiac remodeling, and inhibiting the RAAS is a pharmacological target in heart failure management. As a result, neurohumoral modulation is a crucial treatment principle for managing heart failure. This approach involves using medications like ACE inhibitors (ACEIs), angiotensin receptor blockers (ARBs), β-blockers, mineralocorticoid receptor antagonists (MRAs), and neutral...
690

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Blueberry Bagasse-Enriched Whey Fermented Formulations: Effect of Incorporation Timing on Functional Properties and Neurobiological Evaluation in a Murine Model Using a Selected Formulation.

Nutrients·2026
Same author

Novel Pharmacological and Nanotechnology-Based Therapeutic Strategies for MASLD.

Pharmaceutics·2026
Same author

Developing a core outcome set for the NeuroPoint Alliance Quality Outcomes Database Pediatric Tumor Surgery Registry: a modified Delphi study.

Journal of neuro-oncology·2026
Same author

Binational patterns in use of Food is Medicine among Mexican immigrants.

Frontiers in nutrition·2026
Same author

Oropouche virus RNA persistence and antibody kinetics: a multi-specimen, genomic and serological analysis.

Journal of travel medicine·2026
Same author

Generative AI for climate governance and acceptability-constrained policy design.

npj climate action·2026

Related Experiment Video

Updated: Nov 30, 2025

Renal Ischaemia Reperfusion Injury: A Mouse Model of Injury and Regeneration
12:27

Renal Ischaemia Reperfusion Injury: A Mouse Model of Injury and Regeneration

Published on: June 7, 2014

50.7K

Opioid Preconditioning Modulates Repair Responses to Prevent Renal Ischemia-Reperfusion Injury.

Adriana Franco-Acevedo1, Raquel Echavarria2, Bibiana Moreno-Carranza3

  • 1Doctorado en Farmacologia, Universidad de Guadalajara, Guadalajara 44340, Mexico.

Pharmaceuticals (Basel, Switzerland)
|November 18, 2020
PubMed
Summary

Opioid preconditioning with morphine or fentanyl reduced acute kidney injury from ischemia-reperfusion injury (IRI) in rats. This protective effect involved modulating molecules crucial for tissue repair and new blood vessel growth (angiogenesis).

Keywords:
angiogenesisfentanylmorphineopioidsrenal ischemia-reperfusionwound healing

More Related Videos

Remote Limb Ischemic Preconditioning: A Neuroprotective Technique in Rodents
07:52

Remote Limb Ischemic Preconditioning: A Neuroprotective Technique in Rodents

Published on: June 2, 2015

12.3K
Technical Refinement of a Bilateral Renal Ischemia-Reperfusion Mouse Model for Acute Kidney Injury Research
03:13

Technical Refinement of a Bilateral Renal Ischemia-Reperfusion Mouse Model for Acute Kidney Injury Research

Published on: November 3, 2023

2.7K

Related Experiment Videos

Last Updated: Nov 30, 2025

Renal Ischaemia Reperfusion Injury: A Mouse Model of Injury and Regeneration
12:27

Renal Ischaemia Reperfusion Injury: A Mouse Model of Injury and Regeneration

Published on: June 7, 2014

50.7K
Remote Limb Ischemic Preconditioning: A Neuroprotective Technique in Rodents
07:52

Remote Limb Ischemic Preconditioning: A Neuroprotective Technique in Rodents

Published on: June 2, 2015

12.3K
Technical Refinement of a Bilateral Renal Ischemia-Reperfusion Mouse Model for Acute Kidney Injury Research
03:13

Technical Refinement of a Bilateral Renal Ischemia-Reperfusion Mouse Model for Acute Kidney Injury Research

Published on: November 3, 2023

2.7K

Area of Science:

  • Nephrology
  • Pharmacology
  • Regenerative Medicine

Background:

  • Ischemia-reperfusion injury (IRI) can lead to significant renal damage due to impaired tissue repair mechanisms.
  • Opioid-based anesthetic preconditioning has demonstrated protective effects in myocardial IRI models.
  • Understanding molecular pathways in renal IRI is crucial for developing therapeutic strategies.

Purpose of the Study:

  • To investigate the impact of opioid pharmacological preconditioning on renal function following IRI.
  • To analyze the expression of key molecules involved in renal tissue repair and angiogenesis after opioid preconditioning.
  • To evaluate the potential of opioids in mitigating renal damage in IRI models.

Main Methods:

  • Male rats underwent 45 minutes of renal ischemia followed by 24 hours of reperfusion.
  • Preconditioning involved 60 minutes of morphine or fentanyl administration before ischemia.
  • Assessed serum creatinine, KIM-1 expression, VEGF levels, and performed immunohistochemistry for HIF-1 and Cathepsin D (CTD).
  • Real-time PCR quantified angiogenic genes: HIF-1α, VEGF, VEGF Receptor 2 (VEGF-R2), CTD, CD31, and IL-6.

Main Results:

  • Opioid preconditioning significantly decreased acute kidney injury, restoring renal function to levels comparable to sham controls.
  • Both morphine and fentanyl modulated the expression of HIF-1, VEGF, VEGF-R2, and CD31.
  • Preconditioning negatively regulated Cathepsin D (CTD) expression.
  • Opioid preconditioning reduced IRI-induced injury by modulating the expression of angiogenic molecules.

Conclusions:

  • Pharmacological preconditioning with opioids offers protection against renal IRI.
  • Opioid preconditioning influences key molecular pathways regulating tissue repair and angiogenesis in the kidney.
  • These findings highlight the potential of opioid preconditioning as a therapeutic strategy in renal pathophysiological and surgical contexts.