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Updated: Nov 30, 2025

Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA
Published on: September 8, 2021
Navigating the crowd: visualizing coordination between genome dynamics, structure, and transcription
Haitham A Shaban1,2, Roman Barth3, Kerstin Bystricky4,5
1Spectroscopy Department, Physics Division, National Research Centre, Dokki, Cairo, 12622, Egypt. h_shaban@aucegpt.edu.
Abstract:
The eukaryotic genome is hierarchically structured yet highly dynamic. Regulating transcription in this environment demands a high level of coordination to permit many proteins to interact with chromatin fiber at appropriate sites in a timely manner. We describe how recent advances in quantitative imaging techniques overcome caveats of sequencing-based methods (Hi-C and related) by enabling direct visualization of transcription factors and chromatin at high resolution, from single genes to the whole nucleus. We discuss the contribution of fluorescence imaging to deciphering the principles underlying this coordination within the crowded nuclear space in living cells and discuss challenges ahead.
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