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lncRNA SNHG1 Promotes Progression of Cervical Cancer Through miR-195/NEK2 Axis
Yuan Yuan Ji1, Man Meng2, Ye Miao3
1Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, People's Republic of China.
Objective:
Cervical cancer is a common gynecologic cancer, and no study has been reported on the way through which lncRNA SNHG1, miR-195 and NEK2 jointly affect cervical cancer cells (CCCs), so this paper will explore a new approach to the development of cervical cancer in this respect.
Methods:
Altogether 72 cervical cancer tissues and 54 adjacent tissues were collected. qPCR was performed to quantify lncRNA SNHG1 and miR-195, whose expression vectors were constructed and then transfected into CCCs, so as to observe their effects on the cells. Western blotting (WB) was carried out to detect protein levels. MTT assay was conducted to detect cell activity. Flow cytometry was performed to detect cell apoptosis. Transwell was carried out to detect cell invasion and migration.
Results:
The expression of lncRNA SNHG1 up-regulated while that of miR-195 down-regulated in CCCs. lncRNA SNHG1 regulated NEK2 through its targeted binding to miR-195. The down-regulation of lncRNA SNHG1 or the up-regulation of miR-195 led to the decrease of NEK2 and the reduction of cells' activity, migration and invasion, also resulting in the increase of cell apoptosis. Rescue experiments showed that the down-regulation of miR-195 could offset the cell changes caused by lncRNA SNHG1.
Conclusion:
lncRNA SNHG1 promotes the progression of cervical cancer through the miR-195/NEK2 axis, so lncRNA SNHG1, miR-195 and NEK2 may have potential values for diagnosing and treating cervical cancer.
Insights
Long non-coding RNA SNHG1 promotes cervical cancer progression by targeting miR-195 and regulating NEK2. This pathway affects cell activity, apoptosis, invasion, and migration, offering potential diagnostic and therapeutic targets.
Area of Science:
- Gynecologic Oncology
- Molecular Biology
- Cancer Research
Background:
- Cervical cancer is a prevalent gynecologic malignancy.
- The molecular mechanisms driving cervical cancer progression require further elucidation.
- The roles of long non-coding RNA SNHG1, miR-195, and NEK2 in cervical cancer remain underexplored.
Purpose of the Study:
- To investigate the functional interplay between lncRNA SNHG1, miR-195, and NEK2 in cervical cancer cells.
- To explore the potential of these molecules as diagnostic and therapeutic targets for cervical cancer.
Main Methods:
- Quantitative real-time PCR (qPCR) to assess lncRNA SNHG1 and miR-195 expression.
- Cell transfection with expression vectors for lncRNA SNHG1 and miR-195.
- Western blotting (WB) for protein level analysis.
- MTT assay for cell activity, flow cytometry for apoptosis, and Transwell assays for invasion and migration.
Main Results:
- lncRNA SNHG1 was upregulated, while miR-195 was downregulated in cervical cancer tissues.
- lncRNA SNHG1 directly targeted miR-195, influencing NEK2 expression.
- Downregulation of lncRNA SNHG1 or upregulation of miR-195 reduced NEK2 levels, cell activity, migration, and invasion, while increasing apoptosis.
- Rescue experiments confirmed miR-195's role in mediating lncRNA SNHG1's effects.
Conclusions:
- lncRNA SNHG1 promotes cervical cancer progression via the miR-195/NEK2 axis.
- lncRNA SNHG1, miR-195, and NEK2 represent potential biomarkers and therapeutic targets for cervical cancer.
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