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Baohuoside I via mTOR Apoptotic Signaling to Inhibit Glioma Cell Growth

Yangyang Guo1, Cheng Wang1, Minghui Jiang1

  • 1Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, Zhejiang Provincial Top Key Discipline in Surgery, Wenzhou Medical University First Affiliated Hospital, Wenzhou, Zhejiang, People's Republic of China.

Cancer Management and Research
|November 18, 2020
PubMed
Abstract

Insights

Baohuoside I, a natural compound, effectively inhibits glioma cell proliferation, migration, and invasion by inducing apoptosis through the mTOR signaling pathway. This natural product shows promise as a potential anti-glioma therapeutic agent.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Baohuoside I exhibits anti-cancer properties across various cancers.
  • Its specific role and molecular mechanisms in glioma remain largely unexplored.

Purpose of the Study:

  • To investigate the anti-glioma effects of Baohuoside I.
  • To elucidate the molecular mechanisms underlying its anti-cancer activity in glioma cells.

Main Methods:

  • Cell proliferation was assessed using RTCA, CCK-8, Ki67 immunofluorescence, and colony formation assays.
  • Invasion and migration were evaluated by Transwell and scratch assays.
  • Apoptosis was detected via flow cytometry, and protein expression was analyzed using Western blotting.

Main Results:

  • Baohuoside I significantly inhibited glioma cell proliferation and induced apoptosis.
  • The compound markedly reduced glioma cell migration and invasion.
  • Western blot analysis revealed upregulation of p-AMPKα1 and downregulation of p-mTOR and p-S6K, indicating mTOR pathway involvement.
  • In vivo studies in nude mice demonstrated Baohuoside I's anti-glioma efficacy.

Conclusions:

  • Baohuoside I demonstrates potent anti-glioma activity by inhibiting proliferation, invasion, and migration.
  • The anti-tumor effect is mediated by inducing apoptosis, not autophagy.
  • Baohuoside I targets the mTOR signaling pathway to induce cell apoptosis, positioning it as a potential anti-cancer candidate.
Keywords:
Baohuoside IapoptosisgliomamTORproliferation

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