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Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions
Published on: January 20, 2022
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Urinary glycoproteins associated with aggressive prostate cancer
Mingming Dong1, T Mamie Lih1, Shao-Yung Chen1,2
1Department of Pathology, School of Medicine, Johns Hopkins University, Baltimore, Maryland 21231, USA.
Theranostics
|November 18, 2020
Summary
Researchers identified urinary glycoproteins to detect aggressive prostate cancer. A panel including urinary ACPP, CLU, and serum PSA shows promise as a noninvasive biomarker for aggressive disease detection.
Area of Science:
- Urology
- Proteomics
- Biomarker Discovery
Background:
- Aggressive prostate cancer detection requires improved methods.
- Urine contains secretory glycoproteins useful for noninvasive diagnostics.
- Glycoproteins are crucial in cancer development and progression.
Purpose of the Study:
- Identify urinary glycoproteins differentiating aggressive from non-aggressive prostate cancer.
- Evaluate the diagnostic performance of identified urinary glycoproteins.
- Develop predictive models for aggressive prostate cancer detection.
Main Methods:
- Quantitative mass spectrometry of glycopeptides from urine specimens.
- Discovery cohort: 74 aggressive (Gleason ≥8) and 68 non-aggressive (Gleason = 6) prostate cancer samples.
- Validation of predictive models using a separate validation cohort.
Main Results:
- Urinary ACPP showed superior performance in distinguishing aggressive prostate cancer.
- A panel of urinary ACPP, urinary CLU, and serum PSA achieved an AUC of 0.86.
- A second panel with urinary ACPP, urinary LOX, and serum PSA achieved an AUC of 0.82.
- Consistent performance was observed in the validation cohort.
Conclusions:
- Identified urinary glycoprotein glycopeptides associated with aggressive prostate cancer.
- Demonstrated potential of urinary glycoproteins as noninvasive biomarkers.
- Urinary glycoprotein panels show promise for aggressive prostate cancer detection, warranting multi-center validation.
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