Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Which animals are at risk? Predicting species susceptibility to Covid-19.

bioRxiv : the preprint server for biology·2020
Same author

Human dendritic cell sequestration onto the Necator americanus larval sheath during ex-sheathing: a possible mechanism for immune privilege.

Parasitology·2018
Same author

Probing the causes of thermal hysteresis using tunable <i>N</i><sub>agg</sub> micelles with linear and brush-like thermoresponsive coronas.

Polymer chemistry·2017
Same author

Gold nanoparticle interactions with endothelial cells cultured under physiological conditions.

Biomaterials science·2017
Same author

Discrimination between lectins with similar specificities by ratiometric profiling of binding to glycosylated surfaces; a chemical 'tongue' approach.

RSC advances·2016
Same author

Engineering serendipity: High-throughput discovery of materials that resist bacterial attachment.

Acta biomaterialia·2015

Related Experiment Video

Updated: Nov 29, 2025

Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface
13:36

Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface

Published on: October 20, 2023

3.9K

Developing immune-regulatory materials using immobilized monosaccharides with immune-instructive properties.

M A Alobaid1, S-J Richards2, M R Alexander3

  • 1Immunology & Immuno-Bioengineering, School of Life Sciences, Faculty of Medicine and Health Sciences, University of Nottingham, Nottingham, NG7 2RD, United Kingdom.

Materials Today. Bio
|November 18, 2020
PubMed
Summary

Simple sugars can reprogram immune cells to reduce inflammation. This study shows immobilized monosaccharides can induce regulatory immune cell phenotypes, offering new therapeutic avenues for autoimmune diseases and regenerative medicine.

Keywords:
(Gal1), 100% 1-amino-1-deoxy-β-d-galactose(Gal1–Gal2), 50% 1-amino-1-deoxy-β-d-galactose + 50% 2-amino-2-deoxy-β-d-galactose(Gal2), 100% 2-amino-2-deoxy-β-d-galactose(Gal2–Man1), 90% 2-amino-2-deoxy-β-d-galactose + 10% 1-amino-1-deoxy-β-d-mannose(Gal2–Man2), 2-amino-2-deoxy-β-d-galactose + 10% 2-amino-2-deoxy-β-d-mannose(Man1–Man2), 40% 1-amino-1-deoxy-β-d-mannose + 60% 2-amino-2-deoxy-β-d-mannoseCLR, C-type lectin receptorCarbohydratesDC-SIGN, Dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrinDCs, Dendritic cellsDendritic cellsFBS, Fetal bovine serumFucoseGalactoseIDO, Indoleamine 2,3-dioxygenaseImmune modulationImmune-instructive materialsLPS, LipopolysaccharideMFI, Median fluorescence intensityMR, Mannose receptorMT, 1-methyl-DL-tryptophanMannosePRR, Pattern recognition receptorPolymersT cells

More Related Videos

High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles
14:37

High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles

Published on: July 6, 2012

11.7K
In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
08:02

In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction

Published on: January 22, 2020

5.7K

Related Experiment Videos

Last Updated: Nov 29, 2025

Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface
13:36

Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface

Published on: October 20, 2023

3.9K
High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles
14:37

High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles

Published on: July 6, 2012

11.7K
In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
08:02

In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction

Published on: January 22, 2020

5.7K

Area of Science:

  • Immunology
  • Carbohydrate Chemistry
  • Regenerative Medicine

Background:

  • Dendritic cells (DCs) are key regulators of adaptive immunity, making them targets for immune modulation.
  • Carbohydrates influence DC function, but the effects of simple monosaccharides are not fully understood.
  • Immune modulation strategies are crucial for treating autoimmune diseases, allergies, cancer, and in regenerative medicine.

Purpose of the Study:

  • To investigate how immobilized monosaccharides modulate dendritic cell (DC) phenotype and function.
  • To determine the impact of monosaccharide-induced DC changes on downstream adaptive immune responses.
  • To explore the potential of monosaccharides as tools for immune modulation.

Main Methods:

  • Utilized a combinatorial array of immobilized monosaccharides to treat DCs.
  • Assessed DC activation markers (CD40 expression, IL-12, indoleamine 2,3-dioxygenase activity, IL-10 production).
  • Performed DC-T cell co-cultures to evaluate T cell polarization (Treg, Th17).

Main Results:

  • Specific monosaccharides suppressed lipopolysaccharide-induced DC activation.
  • Monosaccharides induced an anti-inflammatory/regulatory DC phenotype, characterized by reduced CD40, IL-12, and IDO activity, and increased IL-10.
  • DCs cultured on regulatory monosaccharides promoted naïve T cell differentiation towards a regulatory phenotype.
  • Certain monosaccharides induced mixed Treg and Th17 cell differentiation.

Conclusions:

  • Immobilized monosaccharides can effectively prime DCs and skew T cell differentiation towards an immune-regulatory phenotype.
  • Fine-tuning immune responses with simple carbohydrate combinations offers novel immune modulation tools.
  • Potential applications include regenerative medicine, implantable devices, and wound healing by reducing inflammation and promoting homeostasis.