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Updated: Nov 29, 2025
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Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control
Published on: April 11, 2013
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Pattern-triggered immunity restricts host colonization by endophytic fusaria, but does not affect endophyte-mediated
Francisco J de Lamo1, Margarita Šimkovicová1, David H Fresno1
1Molecular Plant Pathology, Faculty of Science, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, Netherlands.
Molecular Plant Pathology
|November 18, 2020
Summary
Endophyte-mediated resistance (EMR) in plants does not rely on pattern-triggered immunity (PTI). However, PTI is crucial for limiting the colonization of plant roots by nonpathogenic Fusarium oxysporum (Fo) isolates.
Area of Science:
- Plant Pathology
- Plant-Microbe Interactions
- Molecular Plant Immunity
Background:
- Fusarium oxysporum (Fo) is a versatile fungus, acting as both a destructive pathogen and a beneficial root endophyte.
- Endophyte-mediated resistance (EMR) is a plant defense mechanism induced by endophytic Fo strains, but its underlying mechanisms are not fully understood.
- EMR is hypothesized to involve pattern-triggered immunity (PTI), a core plant defense system recognizing microbial patterns.
Purpose of the Study:
- To investigate the role of PTI in controlling endophytic Fo colonization.
- To determine if PTI is required for EMR induction against the tomato pathogen Fusarium oxysporum f. sp. lycopersici (Fol).
Main Methods:
- Utilized transgenic tomato and Arabidopsis plants with compromised PTI responses (due to Avr2 effector expression).
- Challenged these plants with an EMR-inducing Fo endophyte (Fo47) and a nonhost pathogen (Fom).
- Assessed host colonization by Fo isolates and EMR induction against Fol.
Main Results:
- PTI-compromised plants showed increased colonization by endophytic and nonhost Fo strains.
- Surprisingly, EMR induction against Fol was not affected in PTI-compromised plants.
- These findings indicate EMR is independent of PTI-mediated defense pathways.
Conclusions:
- Plant defense against pathogenic Fusarium relies on PTI, which restricts nonpathogenic isolate colonization.
- Endophyte-mediated resistance (EMR) operates independently of the conventional PTI pathway.
- This research clarifies the distinct roles of PTI and EMR in plant-microbe interactions.
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