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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Flow cytometric method transfer: Recommendations for best practice.

Maciej Cabanski¹, Teri Oldaker², Jennifer J Stewart³

¹Novartis Institutes for Biomedical Research, Novartis Pharma AG, Basel, Switzerland.

Cytometry. Part B, Clinical Cytometry

|November 18, 2020

Summary

This summary is machine-generated.

Transferring flow cytometry methods requires specific considerations due to complex instrumentation and cellular measurands. This paper outlines best practices for successful flow cytometry method transfer, ensuring reliable data.

Keywords:

design control flow cytometry method transfer technology transfer validation

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Area of Science:

Clinical laboratory science
Biotechnology
Immunology

Background:

Traditional method transfer protocols are insufficient for flow cytometry due to instrument complexity, cellular measurands, lack of reference materials, and specimen instability.
Diverse reasons for method transfer, regulatory settings, and contexts of use necessitate tailored approaches.

Purpose of the Study:

To provide recommendations and best practices for the method transfer of flow cytometry assays.
To highlight the critical impact of proper method transfer on the reliability of flow cytometry data.

Main Methods:

Review of existing method transfer principles and their limitations in flow cytometry.
Development of specific considerations and best practices tailored to flow cytometry.
Inclusion of case studies to illustrate practical application.

Main Results:

Identified key challenges in flow cytometry method transfer, including instrumentation, measurands, reference materials, and specimen stability.
Proposed a framework for best practices in flow cytometry method transfer.
Demonstrated the importance of method transfer for data integrity through case studies.

Conclusions:

Standard method transfer guidelines are inadequate for flow cytometry.
A customized approach focusing on specific challenges is essential for successful flow cytometry method transfer.
Effective method transfer is crucial for ensuring the accuracy and reliability of flow cytometry results.