Biotoxic effects and gene expression regulation of urban PM_2.5 in southwestern China

Atmospheric fine particulate matter (PM_2.5) causes severe haze in China and is regarded as a threat to human health. The health effects of PM_2.5 vary location by location due to the variation in size distribution, chemical composition, and sources. In this study, the cytotoxicity effect, oxidative stress, and gene expression regulation of PM_2.5 in Chengdu and Chongqing, two typical urban areas in southern China, were evaluated. Urban PM_2.5 in summer and winter significantly inhibited cell viability and increased reactive oxygen species (ROS) levels in A549 cells. Notably, PM_2.5 in winter exhibited higher cytotoxicity and ROS level than summer. Moreover, in this study, PM_2.5 commonly induced cancer-related gene expression such as cell adhesion molecule 1 (PECAM1), interleukin 24 (IL24), and cytochrome P450 (CYP1A1); meanwhile, PM_2.5 commonly acted on cancer-related biological functions such as cell-substrate junction, cell-cell junction, and focal adhesion. In particular, PM_2.5 in Chengdu in summer had the highest carcinogenic potential among PM_2.5 at the two sites in summer and winter. Importantly, cancer-related genes were uniquely targeted by PM_2.5, such as epithelial splicing regulatory protein 1 (ESRP1) and membrane-associated ring-CH-type finger 1 (1-Mar) by Chengdu summer PM_2.5; collagen type IX alpha 3 chain (COL9A3) by Chengdu winter PM_2.5; SH2 domain-containing 1B (SH2D1B) by Chongqing summer PM_2.5; and interleukin 1 receptor-like 1 (IL1RL1) and zinc finger protein 42 (ZNF423) by Chongqing winter PM_2.5. Meanwhile, important cancer-related biological functions were specially induced by PM_2.5, such as cell cycle checkpoint by Chengdu summer PM_2.5; macromolecule methylation by Chengdu winter PM_2.5; endoplasmic reticulum-Golgi intermediate compartment membrane by Chongqing summer PM_2.5; and cellular lipid catabolic process by Chongqing winter PM_2.5. Conclusively, in the typical urban areas of southern China, both summer and winter PM_2.5 illustrated significant gene regulation effects. This study contributes to evaluating the adverse health effects of PM_2.5 in southern China and providing public health suggestions for policymakers.