Anatomically induced changes in rice leaf mesophyll conductance explain the variation in photosynthetic nitrogen use...

The ratio of CO₂ mesophyll conductance (g_m) to Ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) content has been suggested to positively affect photosynthetic nitrogen use efficiency (PNUE). The anatomical basis of g_m has been quantified, but information on the relationship between cell-level anatomies and PNUE is less advanced. Here, hydroponic experiments were conducted in rice plants supplied with ammonium (NH₄⁺) and nitrate (NO₃^-) under three N levels (low, 0.71 mM; intermediate, 2.86 mM; high, 7.14 mM) to investigate the gas exchange parameters, leaf anatomical structure and PNUE.

The results showed a lower PNUE in plants supplied with high nitrogen and NH₄⁺, which was positively correlated with the g_m/Rubisco ratio. A one-dimensional within-leaf model revealed that the resistance to CO₂ diffusion in the liquid phase (r_liq) dominated the overall mesophyll resistance (r_m), in which CO₂ transfer resistance in the cell wall, cytoplasm and stroma were significantly affected by nitrogen supply. The chloroplast surface area exposed to intercellular space (S_c) per Rubisco rather than the g_m/S_c ratio was positively correlated with PNUE and was thus considered a key component influencing PNUE.

In conclusion, our study emphasized that S_c was the most important anatomical trait in coordinating g_m and PNUE with contrasting N supply.