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IFITM proteins inhibit the late step of feline foamy virus replication

Jinsun Kim1, Cha-Gyun Shin1

  • 1Department of Systems Biotechnology, Chung-Ang University, Ansung, Republic of Korea.

Animal Cells and Systems
|November 19, 2020
PubMed

Insights

Interferon-induced transmembrane (IFITM) proteins restrict feline foamy virus (FFV) replication. IFITMs inhibit FFV late-stage replication, reducing viral production and Gag protein levels.

Area of Science:

  • Virology
  • Cell Biology
  • Immunology

Background:

  • Interferon-induced transmembrane (IFITM) proteins are known host restriction factors.
  • IFITMs inhibit the replication of various viruses.
  • The role of IFITMs in feline foamy viral (FFV) replication is not well understood.

Purpose of the Study:

  • To investigate whether IFITM proteins inhibit FFV replication.
  • To determine the specific stage of the FFV replication cycle inhibited by IFITMs.

Main Methods:

  • Transient IFITM expression vectors were utilized.
  • Cells were infected with FFV at various time points relative to IFITM transfection.
  • Viral production and Gag protein levels were quantified.

Main Results:

  • Viral production was significantly reduced when FFV infection occurred shortly after IFITM transfection.
  • IFITM expression did not inhibit viral production when maximized at later time points post-transfection.
  • Viral Gag protein levels correlated with viral titers, suggesting late-stage inhibition.

Conclusions:

  • IFITM proteins restrict FFV replication.
  • IFITMs appear to inhibit a late step in the FFV replication cycle.
  • IFITM-mediated restriction involves the inhibition of viral production and Gag protein accumulation.
Keywords:
IFITMfeline foamy viruslate step

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