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First- and Second-Trimester Reference Intervals for Thyroid Function Testing in a US Population.

Dustin R Bunch1,2, Kyle Firmender1, Roa Harb1,2

  • 1Department of Laboratory Medicine, Yale-New Haven Hospital, New Haven, CT.

American Journal of Clinical Pathology
|November 19, 2020
PubMed
Summary
This summary is machine-generated.

This study establishes trimester-specific reference intervals for thyroid function tests in pregnant individuals using Roche analyzers. These new intervals for thyroid-stimulating hormone (TSH) and other thyroid hormones aid in better pregnancy care.

Keywords:
PregnancyReference intervalsRocheThyroid testing

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Area of Science:

  • Clinical Chemistry
  • Endocrinology
  • Reproductive Medicine

Background:

  • Thyroid dysfunction during pregnancy poses risks to both mother and child.
  • Standard clinical laboratories often lack trimester-specific reference intervals for thyroid function tests.
  • Accurate thyroid hormone levels are crucial for monitoring and managing pregnancy health.

Purpose of the Study:

  • To establish first- and second-trimester specific reference intervals for key thyroid function tests.
  • To provide these intervals for thyroid-stimulating hormone (TSH), free thyroxine (FT4), total thyroxine (T4), and total triiodothyronine (T3).
  • To utilize data from a US population measured on Roche analyzers.

Main Methods:

  • Patient samples were collected from first- and second-trimester prenatal screening.
  • Samples excluded singleton pregnancies with positive thyroid peroxidase or thyroglobulin antibodies.
  • Thyroid analytes were measured using Roche Modular e170 and verified on Roche cobas e801 analyzers.

Main Results:

  • First-trimester reference intervals: TSH (0.16-2.82 mIU/L), FT4 (12.0-18.5 pmol/L), T4 (62.8-177.9 nmol/L), T3 (1.5-3.4 nmol/L).
  • Second-trimester reference intervals: TSH (0.40-3.62 mIU/L), FT4 (10.2-16.6 pmol/L), T4 (66.6-176.0 nmol/L), T3 (1.56-3.6 nmol/L).
  • Established intervals were verified across two Roche analytical platforms.

Conclusions:

  • This study presents the first trimester-specific thyroid function test reference intervals for a US population using Roche analyzers.
  • The established intervals are consistent with global reports, enhancing clinical utility.
  • These findings support improved diagnosis and management of thyroid dysfunction in pregnancy.