Related Experiment Video

Updated: Nov 29, 2025

Y-27632 Enriches the Yield of Human Melanocytes from Adult Skin Tissues
08:06

Y-27632 Enriches the Yield of Human Melanocytes from Adult Skin Tissues

Published on: July 8, 2020

5.8K

miR-617 Promotes the Growth of IL-22-Stimulated Keratinocytes Through Regulating FOXO4 Expression

Tao Liu1, Xiaomei Feng2, Yongmei Liao3

  • 1Department of Dermatology, The Affiliated Hospital of Southwest Medical University, No. 25 of Taiping Road, Luzhou, 646000, Sichuan, China. TaoLiufjk@163.com.

Biochemical Genetics
|November 19, 2020
PubMed

Insights

MicroRNA-617 (miR-617) promotes psoriasis by increasing keratinocyte proliferation via targeting FOXO4. Inhibiting miR-617 may offer a new therapeutic strategy for this chronic inflammatory skin disease.

Area of Science:

  • Dermatology
  • Molecular Biology
  • Genetics

Background:

  • Psoriasis is a chronic inflammatory skin condition with complex pathogenesis.
  • MicroRNAs (miRNAs) play a role in psoriasis development, but miR-617's specific function remains elusive.

Purpose of the Study:

  • To investigate the role of miR-617 in psoriasis pathogenesis.
  • To identify the molecular targets and mechanisms of miR-617 in keratinocytes.

Main Methods:

  • Quantitative reverse transcription-PCR (qRT-PCR) and Western blot to measure miR-617 and FOXO4 levels.
  • Cell counting kit-8 (CCK-8) assay for proliferation, flow cytometry for cell cycle and apoptosis.
  • Dual luciferase assay to confirm the targeting relationship between miR-617 and FOXO4.

Main Results:

  • miR-617 was upregulated in psoriatic tissues and IL-22-stimulated keratinocytes.
  • miR-617 mimics enhanced proliferation and cell cycle, while suppressing apoptosis; inhibitors had opposite effects.
  • FOXO4 was identified as a direct target of miR-617 and was downregulated in psoriasis; FOXO4 overexpression counteracted miR-617's effects.

Conclusions:

  • miR-617 promotes keratinocyte proliferation in IL-22-stimulated cells by targeting FOXO4.
  • This miR-617/FOXO4 axis represents a potential therapeutic target for psoriasis treatment.
Keywords:
Cell growthFOXO4KeratinocytesPsoriasismiR-617

More Related Videos

Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates08:19

Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates

Published on: April 22, 2019

7.3K
Generation and Culturing of Primary Human Keratinocytes from Adult Skin10:42

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Published on: December 22, 2017

17.0K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.2K
Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.8K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
4.8K
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.8K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.3K