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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Nov 29, 2025

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

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Published on: December 16, 2017

Tracking development at the cellular level

¹Laboratory of single-cell genomics and population dynamics, Rockefeller University, New York, NY, USA. jcao@rockefeller.edu.

Science (New York, N.Y.)

|November 20, 2020

Summary

No abstract available in PubMed .

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