Related Experiment Video
Updated: Nov 29, 2025

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
Gen-FS coordinated proficiency test data for genomic foodborne pathogen surveillance, 2017 and 2018 exercises
Ruth E Timme1, Patricia C Lafon2, Maria Balkey3
1US Food and Drug Administration, College Park, MD, USA. ruth.timme@fda.hhs.gov.
The US PulseNet and GenomeTrakr networks now share genomic data for foodborne pathogen surveillance. This collaboration harmonized their 2017-2018 proficiency tests, releasing reference genomes and raw data.
Area of Science:
- Food safety
- Genomics
- Microbiology
Background:
- The US PulseNet and GenomeTrakr networks are key players in foodborne pathogen surveillance.
- Genomics for Food Safety (Gen-FS) consortium facilitates collaboration between these networks.
- Harmonization of genomic data collection and analysis is crucial for effective surveillance.
Purpose of the Study:
- To release reference genomes and raw data from harmonized proficiency testing exercises.
- To support the collaborative efforts of PulseNet and GenomeTrakr within the Gen-FS consortium.
- To enhance the accuracy and comparability of genomic data for foodborne pathogen surveillance.
Main Methods:
- Harmonization of proficiency test exercises between PulseNet and GenomeTrakr starting in 2017.
- Agreement on a single strain-set for proficiency testing.
- Adoption of a standardized operating procedure (SOP) for genomic data collection.
Main Results:
- Successful joint coordination of annual proficiency test exercises in 2017 and 2018.
- Publication of reference genomes from these exercises.
- Availability of raw data submissions for the 2017 and 2018 proficiency tests.
Conclusions:
- The harmonization of proficiency tests strengthens collaborative foodborne pathogen surveillance.
- The released genomic data provides valuable resources for research and public health.
- Standardized methods improve the reliability of genomic surveillance data.
Related Concept Videos
Genomics
Microorganisms in Agriculture and Food industry
Stringent Response in E. coli
Principles of Disease Surveillance
Genomic DNA in Prokaryotes
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genome-wide Association Studies-GWAS
GWAS does not require the identification of the target gene involved in...

