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Related Experiment Video

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Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing

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Integrative quantitative-phase and airy light-sheet imaging.

N R Subedi¹, P S Jung^2,3, E L Bredeweg⁴

¹Department of Physics, University of Idaho, Moscow, ID, 83844, USA.

Scientific Reports

|November 20, 2020

Summary

This summary is machine-generated.

We fused light-sheet microscopy and quantitative-phase imaging for label-free cell analysis. This novel system reveals how cellular noise impacts metabolic reaction localization in live cells.

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Area of Science:

Biophysics
Cell Biology
Microscopy

Background:

Light-sheet microscopy offers speed and reduced phototoxicity.
Quantitative-phase imaging provides label-free cell and organelle analysis.
Cellular number-density is a thermodynamic indicator of metabolism.

Purpose of the Study:

To integrate light-sheet microscopy and quantitative-phase imaging onto a standard inverted microscope.
To enable label-free, quantitative analysis of live cells in microfluidics.
To investigate the impact of cellular noise on metabolic reaction localization.

Main Methods:

Fusion of quantitative-phase imaging with an accelerating Airy-beam light-sheet.
Utilized a standard inverted microscope compatible with microfluidics and open-source software.
Performed multivariate investigations on live cells.

Main Results:

Achieved imaging areas over an order of magnitude larger than Gaussian beams.
Demonstrated the system's capability to image live cells in microfluidics.
Unmasked the influence of cellular noise on metabolic reaction compartmentalization.

Conclusions:

The integrated imaging system provides a powerful tool for live-cell analysis.
Revealed novel insights into the relationship between cellular noise and metabolism.
Potential applications in biotechnology and evolutionary biology.