Integrative quantitative-phase and airy light-sheet imaging
N R Subedi1, P S Jung2,3, E L Bredeweg4
1Department of Physics, University of Idaho, Moscow, ID, 83844, USA.
Scientific Reports
|November 20, 2020
Summary
We fused light-sheet microscopy and quantitative-phase imaging for label-free cell analysis. This novel system reveals how cellular noise impacts metabolic reaction localization in live cells.
Area of Science:
- Biophysics
- Cell Biology
- Microscopy
Background:
- Light-sheet microscopy offers speed and reduced phototoxicity.
- Quantitative-phase imaging provides label-free cell and organelle analysis.
- Cellular number-density is a thermodynamic indicator of metabolism.
Purpose of the Study:
- To integrate light-sheet microscopy and quantitative-phase imaging onto a standard inverted microscope.
- To enable label-free, quantitative analysis of live cells in microfluidics.
- To investigate the impact of cellular noise on metabolic reaction localization.
Main Methods:
- Fusion of quantitative-phase imaging with an accelerating Airy-beam light-sheet.
- Utilized a standard inverted microscope compatible with microfluidics and open-source software.
- Performed multivariate investigations on live cells.
Main Results:
- Achieved imaging areas over an order of magnitude larger than Gaussian beams.
- Demonstrated the system's capability to image live cells in microfluidics.
- Unmasked the influence of cellular noise on metabolic reaction compartmentalization.
Conclusions:
- The integrated imaging system provides a powerful tool for live-cell analysis.
- Revealed novel insights into the relationship between cellular noise and metabolism.
- Potential applications in biotechnology and evolutionary biology.
