Related Experiment Video
Updated: Nov 29, 2025

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
Published on: July 22, 2016
Hypothetical Proteins as Predecessors of Long Non-coding RNAs
Girik Malik1, Tanu Agarwal1, Utkarsh Raj1
11Khoury College of Computer Sciences, Northeastern University, 360 Huntington Ave., Boston, MA02115, USA; 2Bioclues.org, Kukatpally, Hyderabad, 500072, India; 3Labrynthe Pvt. Ltd., New Delhi, India; 4NIIT University, NH8, Delhi- Jaipur Highway, District Alwar, Neemrana, Rajasthan 301705, India; 5Hopp Children's Cancer Center [KiTZ], Heidelberg, Germany; 6Division of Pediatric Neuro Oncology, German Cancer Research Center [DKFZ], German Cancer Consortium [DKTK], Heidelberg, Germany; 7Heidelberg University, Medical Faculty, Heidelberg, Germany; 8Department of Biotechnology and Bioinformatics, Birla Institute of Scientific Research, Statue Circle, Jaipur302021, RJ, India.
Abstract:
Hypothetical Proteins [HP] are the transcripts predicted to be expressed in an organism, but no evidence of it exists in gene banks. On the other hand, long non-coding RNAs [lncRNAs] are the transcripts that might be present in the 5' UTR or intergenic regions of the genes whose lengths are above 200 bases. With the known unknown [KU] regions in the genomes rapidly existing in gene banks, there is a need to understand the role of open reading frames in the context of annotation. In this commentary, we emphasize that HPs could indeed be the predecessors of lncRNAs.
Related Concept Videos
lncRNA - Long Non-coding RNAs
lncRNA - Long Non-coding RNAs
Types of RNA
RNA Performs Diverse...
Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Ribosomal RNA Synthesis
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Alternative RNA Splicing
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

