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Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Detection of Bacteria Using Fluorogenic DNAzymes

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Published on: May 28, 2012

Fluorescent Probes for Ecto-5'-nucleotidase (CD73).

Constanze C Schmies¹, Georg Rolshoven¹, Riham M Idris¹

¹PharmaCenter Bonn, Pharmaceutical Institute, Department of Pharmaceutical & Medicinal Chemistry, University of Bonn, An der Immenburg 4, D-53121 Bonn, Germany.

ACS Medicinal Chemistry Letters

|November 20, 2020

Summary

This summary is machine-generated.

Researchers developed novel fluorescent CD73 inhibitors. These high-affinity probes, derived from PSB-12379, are valuable for studying the immunosuppressive enzyme ecto-5'-nucleotidase (CD73) in biological and diagnostic applications.

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Area of Science:

Biochemistry
Medicinal Chemistry
Immunology

Background:

Ecto-5'-nucleotidase (CD73) generates adenosine, an immunosuppressive molecule.
CD73 is prevalent in cancer cells, promoting an immunosuppressive tumor microenvironment.

Purpose of the Study:

To design and synthesize novel fluorescent inhibitors of CD73.
To create high-affinity probes for CD73 for biological and diagnostic use.

Main Methods:

Chemical synthesis of fluorescent-labeled CD73 inhibitors based on N-benzyl-α,β-methylene-ADP (PSB-12379).
Characterization of inhibitor affinity and selectivity for CD73.

Main Results:

Developed fluorescent CD73 inhibitors with low nanomolar affinity.
Identified PSB-18332 (2.98 nM) and PSB-19416 (12.6 nM) as high-affinity probes.
Achieved high selectivity for CD73.

Conclusions:

The synthesized fluorescent probes are effective tools for CD73 research.
These compounds can be utilized in biological studies, drug screening, and diagnostics.