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LncRNA-TMPO-AS1 promotes apoptosis of osteosarcoma cells by targeting miR-329 and regulating E2F1

X Liu1, H Wang, G-L Tao

  • 1Department of Laboratory, Second Affiliated Hospital of Dalian Medical University, Dalian, China. liulili198102@163.com.

European Review for Medical and Pharmacological Sciences
|November 20, 2020
PubMed
Abstract

Insights

Long non-coding RNA TMPO-AS1 is elevated in osteosarcoma and serves as a diagnostic marker. Targeting TMPO-AS1 promotes osteosarcoma cell apoptosis via the miR-329/E2F1 pathway, offering a potential therapeutic strategy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Osteosarcoma is a primary bone malignancy with limited treatment options.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development and progression.
  • The specific role and mechanism of lncRNA-TMPO-AS1 in osteosarcoma remain largely unexplored.

Purpose of the Study:

  • To investigate the clinical significance of lncRNA-TMPO-AS1 in osteosarcoma diagnosis and prognosis.
  • To elucidate the molecular mechanism underlying the function of lncRNA-TMPO-AS1 in osteosarcoma progression.
  • To explore the potential of targeting lncRNA-TMPO-AS1 as a therapeutic strategy for osteosarcoma.

Main Methods:

  • Analysis of TMPO-AS1 expression in osteosarcoma tissues and adjacent normal tissues from 51 patients.
  • In vitro functional assays including MTT, Transwell invasion, and flow cytometry to assess cell proliferation, invasion, and apoptosis.
  • Luciferase reporter assays to confirm the targeting relationships between TMPO-AS1, miR-329, and E2F1.
  • Cell transfection with vectors for gene overexpression or inhibition.

Main Results:

  • TMPO-AS1 expression was significantly upregulated in osteosarcoma tissues compared to adjacent tissues.
  • Serum TMPO-AS1 demonstrated diagnostic value for osteosarcoma with an AUC > 0.8.
  • Inhibition of TMPO-AS1 or overexpression of miR-329 suppressed osteosarcoma cell proliferation and invasion while promoting apoptosis.
  • TMPO-AS1 targets miR-329, which in turn targets E2F1, regulating epithelial-mesenchymal transition (EMT) in osteosarcoma.

Conclusions:

  • lncRNA-TMPO-AS1 serves as a valuable diagnostic and prognostic biomarker for osteosarcoma.
  • TMPO-AS1 promotes osteosarcoma cell proliferation and invasion by targeting the miR-329/E2F1 axis.
  • Targeting lncRNA-TMPO-AS1 presents a promising therapeutic avenue for osteosarcoma treatment.

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