Loop-mediated isothermal amplification technique: principle, development and wide application in food safety

Area of Science:

• Food safety science
• Molecular biology techniques
• Analytical chemistry

Background:

• Food safety is a critical global challenge impacting public health.
• Novel detection methods are needed to address complex food safety issues.
• Loop-mediated isothermal amplification (LAMP) is a promising technology for rapid and specific detection.

Purpose of the Study:

• To review the principles, development, and advantages of LAMP.
• To explore the integration of LAMP with various platforms (microfluidics, paper-chips, electrochemical devices, nanomaterials, digital devices).
• To comprehensively analyze LAMP applications in food safety, including pathogen, allergen, pesticide, and GMO detection.

Main Methods:

• Review of existing literature on LAMP technology and its applications.
• Systematic analysis of LAMP integration with different analytical devices.
• Comprehensive survey of LAMP's utility in detecting various food contaminants and components.

Main Results:

• LAMP offers rapid, simple, and highly specific gene amplification under isothermal conditions.
• LAMP can be effectively integrated with diverse platforms, enhancing its applicability.
• LAMP has demonstrated significant potential in detecting foodborne pathogens, allergens, organophosphorus pesticides, and GMOs.

Conclusions:

• LAMP is a versatile and powerful tool for advancing food safety detection.
• Further development of integrated LAMP systems will improve sensitivity and on-site detection capabilities.
• LAMP technology is poised to play a crucial role in ensuring global food security and quality.