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Updated: Nov 29, 2025
High-throughput Saccharification Assay for Lignocellulosic Materials
Published on: July 3, 2011
Simultaneous saccharification and lactic acid fermentation of the cellulosic fraction of municipal solid waste using
Micaela G Chacón1, Christopher Ibenegbu1, David J Leak2
1Department of Biology and Biochemistry, University of Bath, Bath, BA2 7AY, England, UK.
Objective:
A primary drawback to simultaneous saccharification and fermentation (SSF) processes is the incompatibility of the temperature and pH optima for the hydrolysis and fermentation steps-with the former working best at 50-55 °C and pH 4.5-5.5. Here, nine thermophilic Bacillus and Parageobacillus spp. were evaluated for growth and lactic acid fermentation at high temperature and low pH. The most promising candidate was then carried forward to demonstrate SSF using the cellulosic fraction from municipal solid waste (MSW) as a feedstock.
Results:
B. smithii SA8Eth was identified as the most promising candidate and in a batch SSF maintained at 55 °C and pH 5.0, using a cellulase dose of 5 FPU/g glucan, it produced 5.1 g/L lactic acid from 2% (w/v) MSW cellulosic pulp in TSB media.
Conclusion:
This work has both scientific and industrial relevance, as it evaluates a number of previously untrialled bacterial hosts for their compatibility with lignocellulosic SSF for lactic acid production and successfully identifies B. smithii as a potential candidate for such a process.
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