Related Experiment Video
Updated: Nov 29, 2025

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
Published on: January 25, 2020
Novel orthogonal methods to uncover the complexity and diversity of nuclear architecture
Sjoerd Jd Tjalsma1, Wouter de Laat1
1Oncode Institute, Hubrecht Institute-KNAW and University Medical Center Utrecht, 3584 CT Utrecht, The Netherlands.
Abstract:
Recent years have seen a vast expansion of knowledge on three-dimensional (3D) genome organization. The majority of studies on chromosome topology consists of pairwise interaction data of bulk populations of cells and therefore conceals heterogenic and more complex folding patterns. Here, we discuss novel methodologies to study the variation in genome topologies between different cells and techniques that allow analysis of complex, multi-way interactions. These technologies will aid the interpretation of genome-wide chromosome conformation data and provide strategies to further dissect the interplay between genome architecture and transcription regulation.
More Related Videos
Related Concept Videos
Nuclear Overhauser Enhancement (NOE)
Directionality of Nuclear Transport
Atomic Nuclei: Nuclear Spin State Population Distribution
Nuclear Stability
To hold positively charged protons together...
Additional Subnuclear Structures
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles,...
Additional Subnuclear Structures

