Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Centrioles and Centrosomes01:13

Centrioles and Centrosomes

4.6K
Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or...
4.6K
Centrosome Duplication02:25

Centrosome Duplication

4.5K
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
4.5K
Determining the Plane of Cell Division02:13

Determining the Plane of Cell Division

3.6K
Positioning the cell division plane is a critical step during development and cell differentiation, particularly during mitosis when the plane is essential for determining the size of the two daughter cells. The cell division plane is perpendicular to the plane of chromosome segregation, but different types of organisms have different cell division mechanisms to suit their morphology and function. 
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division...
3.6K
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

4.8K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.8K
Spindle Assembly02:50

Spindle Assembly

4.0K
Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
4.0K
Cells Coordinate Growth and Proliferation02:36

Cells Coordinate Growth and Proliferation

4.8K
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
4.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Comprehensive Approach to Monitor Endocytosis and Trafficking of G Protein-Coupled Receptors: Tools for Exploring Receptor-Lipid Interaction.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

An integrated experimental and computational pipeline for crystallographic fragment screening of membrane protein in the lipid cubic phase.

Communications chemistry·2026
Same author

A cryo-EM processing pipeline for microtubules using CryoSPARC.

Acta crystallographica. Section D, Structural biology·2026
Same author

Label-free impedimetric electrochemical aptasensor for the detection of Bacillus anthracis spores using a disposable screen-printed electrode.

Biochemical and biophysical research communications·2026
Same author

Machine Learning Algorithm for Nanomedicine: AI Curated Nanocarriers for Cancer Treatment.

Current pharmaceutical design·2026
Same author

Sustainable development of copper matrix hybrid composites using waste stainless steel chips: a physical and tribological investigation.

Scientific reports·2026

Related Experiment Video

Updated: Nov 29, 2025

Imaging Centrosomes in Fly Testes
09:41

Imaging Centrosomes in Fly Testes

Published on: September 20, 2013

16.3K

Centriole length control.

Ashwani Sharma1, Natacha Olieric1, Michel O Steinmetz2

  • 1Laboratory of Biomolecular Research, Division of Biology and Chemistry, Paul Scherrer Institut, CH-5232 Villigen, Switzerland.

Current Opinion in Structural Biology
|November 21, 2020
PubMed
Summary
This summary is machine-generated.

Researchers propose classifying proteins controlling centriole length into activators (CEAs) and inhibitors (CEIs) based on their microtubule interactions. This framework aids understanding centriole regulation and associated human pathologies.

More Related Videos

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

15.6K
Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles
10:38

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles

Published on: September 21, 2018

9.9K

Related Experiment Videos

Last Updated: Nov 29, 2025

Imaging Centrosomes in Fly Testes
09:41

Imaging Centrosomes in Fly Testes

Published on: September 20, 2013

16.3K
Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

15.6K
Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles
10:38

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles

Published on: September 21, 2018

9.9K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Structural Biology

Background:

  • Centrioles are essential microtubule-based organelles involved in cell division and ciliogenesis.
  • Dysregulation of centriole structure, size, or number is linked to various human diseases.
  • Understanding the molecular mechanisms governing centriole length is crucial for cell biology and medicine.

Purpose of the Study:

  • To propose a novel classification of proteins that regulate centriole length.
  • To elucidate the structure-function relationships and interaction networks of these regulatory proteins.
  • To identify open questions and future research directions in centriole length regulation.

Main Methods:

  • Review and synthesis of existing literature on centriole biogenesis and protein function.
  • Analysis of protein structure-function relationships concerning centriolar microtubules.
  • Mapping of protein interaction networks involved in centriole length control.

Main Results:

  • Proteins controlling centriole length are categorized into two antagonistic classes: centriole elongation activators (CEAs) and centriole elongation inhibitors (CEIs).
  • Detailed discussion of the structure-function dynamics of CEAs and CEIs.
  • Illustration of the complex interaction networks governing centriole length.

Conclusions:

  • The proposed CEA/CEI classification provides a unifying framework for understanding centriole length regulation.
  • This classification facilitates the study of how protein interactions impact centriole homeostasis.
  • Future research should focus on validating these classifications and exploring therapeutic targets for centriole-related pathologies.