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Long noncoding RNA ARRDC1-AS1 is activated by STAT1 and exerts oncogenic properties by sponging miR-432-5p/PRMT5 axis

Xiao-Dong Zou1, Qian Zang2, Zhen-Zhong Zhang1

  • 1Department of Neurology, Tongde Hospital of Zhejiang Provience, Hangzhou, 310012, Zhejiang, China.

Biochemical and Biophysical Research Communications
|November 22, 2020
PubMed

Insights

Long noncoding RNA ARRDC1-AS1 is upregulated in glioma and promotes tumor growth. Inhibiting ARRDC1-AS1 may offer new therapeutic strategies for glioma patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) are implicated in cancer development.
  • Dysregulation of lncRNA ARRDC1-AS1 is observed in various tumors.
  • The role of ARRDC1-AS1 in glioma remains unexplored.

Purpose of the Study:

  • To investigate the expression and function of ARRDC1-AS1 in glioma.
  • To elucidate the underlying molecular mechanisms of ARRDC1-AS1 in glioma progression.
  • To explore potential therapeutic targets for glioma.

Main Methods:

  • Quantitative real-time PCR to measure ARRDC1-AS1 expression in glioma tissues and cell lines.
  • Analysis of the correlation between ARRDC1-AS1 expression and clinical parameters.
  • In vitro functional assays (proliferation, migration, invasion) after ARRDC1-AS1 knockdown.
  • Mechanism studies involving RNA immunoprecipitation and luciferase reporter assays.
  • Rescue experiments to validate the role of the identified molecular axis.

Main Results:

  • ARRDC1-AS1 expression is significantly increased in glioma and correlates with advanced stage and poor prognosis.
  • STAT1 transcriptionally activates ARRDC1-AS1.
  • Knockdown of ARRDC1-AS1 inhibits glioma cell proliferation, migration, and invasion.
  • ARRDC1-AS1 acts as a molecular sponge for miR-432-5p, leading to PRMT5 upregulation.
  • miR-432-5p knockdown reverses the inhibitory effects of ARRDC1-AS1 knockdown.

Conclusions:

  • The STAT1/ARRDC1-AS1/miR-432-5p/PRMT5 axis plays a crucial role in glioma progression.
  • ARRDC1-AS1 is a potential oncogene and therapeutic target in glioma.
  • Targeting this axis may provide novel treatment strategies for glioma.
Keywords:
BiomarkerGliomaLncRNA ARRDC1-AS1PRMT5STAT1miR-432-5p

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