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An apparatus for continuous analysis of protease-catalyzed acyl transfer reactions
V Schellenberger1, U Schellenberger, Y V Mitin
1Sekton Biowissenschaften der Karl-Marx-Universität Leipzig, German Democratic Republic.
Analytical Biochemistry
|September 1, 1987
Abstract:
An apparatus that allows continuous analysis of protease-catalyzed acyl transfer reactions is described. Hydrolysis reaction is assayed using automatic titration. A continuous determination of amino group concentration by reaction with o-phthalaldehyde gives the rate of peptide bond formation. The apparatus allows the determination of the partition constant for the nucleophile at various nucleophile concentrations from one run.