Related Experiment Video
Updated: Nov 29, 2025
07:30
Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
Published on: May 10, 2018
9.5K
High-Throughput Screening for Insulin Secretion Modulators.
1Lilly Diabetes Center of Excellence, Indiana Biosciences Research Institute, Indianapolis, IN, USA. mkalwat@indianabiosciences.org.
Methods in Molecular Biology (Clifton, N.J.)
|November 22, 2020
Summary
This study presents a cost-effective method for screening compounds that affect insulin secretion using novel secreted luciferase tools. This high-throughput screening protocol enables researchers to identify small molecules impacting pancreatic beta cell function.
Area of Science:
- Biochemistry
- Molecular Biology
- Endocrinology
Background:
- High-throughput screening for insulin secretion has been limited by cost and technical hurdles.
- Advancements in secreted luciferase technology now enable accessible screening for small laboratories.
Purpose of the Study:
- To outline methods for high-throughput screening of small molecules impacting pancreatic beta cell function.
- To detail a protocol for assessing chronic effects on insulin secretion.
Main Methods:
- Utilizes secreted luciferase tools for measuring insulin secretion.
- Employs a 384-well plate format for scalability.
- Focuses on optimizing assay dynamic range and reducing variability.
Main Results:
- The protocol allows for processing 48-144 plates weekly.
- Demonstrates feasibility for small laboratories to conduct large-scale compound screening.
Conclusions:
- This high-throughput screening protocol provides a guideline for identifying compounds affecting insulin secretion.
- The method is adaptable and can be improved with emerging technologies.
