Related Experiment Video

Updated: Nov 29, 2025

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
12:21

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

Published on: November 30, 2013

15.2K

Novel MicroRNA Binding Site SNPs and the Risk of Clear Cell Renal Cell Carcinoma (ccRCC): A Case-Control Study

Irina R Gilyazova1, Narasimha M Beeraka2, Elizaveta A Klimentova1

  • 1Institute of Biochemistry and Genetics - Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, Ufa,Russian Federation.

Current Cancer Drug Targets
|November 23, 2020
PubMed
Abstract

Insights

Single-nucleotide polymorphisms (SNPs) in microRNA binding sites of VHL-HIF1α pathway genes are linked to kidney cancer risk and survival. These genetic variations may influence the development and progression of clear cell renal cell carcinoma (ccRCC).

Area of Science:

  • Oncology
  • Genetics
  • Molecular Biology

Background:

  • Renal cell carcinoma (RCC) accounts for 3% of adult malignancies.
  • MicroRNAs (miRNAs) regulate apoptosis and cell cycle control.
  • Single-nucleotide polymorphisms (SNPs) in miRNA-binding sites of VHL-HIF1α pathway genes are potentially associated with renal cancer.

Purpose of the Study:

  • Investigate SNPs affecting miRNA binding sites in VHL-HIF1α pathway genes.
  • Determine the association between these SNPs and clear cell renal cell carcinoma (ccRCC) risk.
  • Evaluate the impact of these variants on patient survival.

Main Methods:

  • Study included 450 ccRCC cases and 490 controls.
  • Genotyping performed using TaqMan PCR allelic discrimination.
  • Kaplan-Meier method used for survival analysis.

Main Results:

  • Polymorphism rs10491534 in TSC1 gene associated with advanced ccRCC risk.
  • VHL gene rs1642742 allele G prevalent in ccRCC patients aged 55+ (OR=1.56).
  • CDCP1 rs6773576 allele A associated with higher mortality risk (HR=3.93, P=0.0033).

Conclusions:

  • miRNA binding site variants in VHL-HIF1α pathway genes are associated with ccRCC risk.
  • These genetic variations may influence ccRCC clinical outcomes and patient survival.
Keywords:
Renal cell carcinomaVHL-HIF1α pathwayepigenetics.miRNApolymorphism

More Related Videos

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.7K
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method

Published on: October 7, 2025

187

Related Concept Videos

Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
17.5K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.5K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.4K
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
12.7K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.5K