Related Experiment Video

Updated: Nov 29, 2025

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
11:42

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

9.7K

UBE2C mRNA expression controlled by miR-300 and HuR determines its oncogenic role in gastric cancer

Ying Wang1, Feifei Huang2, Ming Liu2

  • 1State Key Laboratory of Pharmaceutical Biotechnology, Department of Hematology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, China-Australia Center for Translational Medicine, School of Life Sciences, Nanjing University, Nanjing, 210000, China; Department of Urology, University of California, San Francisco, San Francisco, CA, 94158, USA; Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, San Francisco, CA, 94158, USA.

Biochemical and Biophysical Research Communications
|November 23, 2020
PubMed

Insights

Ubiquitin Conjugating Enzyme E2 C (UBE2C) is overexpressed in gastric cancer, primarily at the mRNA level. Suppressing UBE2C inhibits cancer growth and DNA synthesis, revealing potential therapeutic targets.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Ubiquitin Conjugating Enzyme E2 C (UBE2C) is implicated in oncogenesis across various human cancers.
  • Understanding the precise mechanisms and regulatory pathways of UBE2C in gastric cancer is crucial for therapeutic development.

Purpose of the Study:

  • To investigate the expression levels and alterations of UBE2C in gastric cancer.
  • To identify downstream targets and regulatory mechanisms of UBE2C in gastric cancer progression.
  • To explore the potential of targeting UBE2C for gastric cancer treatment.

Main Methods:

  • Quantitative analysis of UBE2C expression in gastric cancer patients.
  • Experimental manipulation of UBE2C levels (silencing) in gastric cancer cell lines.
  • Assessment of DNA biosynthesis and colony formation assays.
  • MicroRNA expression analysis and RNA binding protein interaction studies.

Main Results:

  • UBE2C is frequently overexpressed in gastric cancer, predominantly via elevated mRNA levels rather than gene amplification.
  • Silencing UBE2C significantly suppresses gastric cancer cell proliferation and inhibits DNA biosynthesis.
  • MicroRNA-300 was identified as a suppressor of gastric cancer progression by reducing UBE2C mRNA abundance, with HuR protecting UBE2C mRNA.
  • Several potential UBE2C-regulated genes contributing to its oncogenic activity were proposed based on expression correlation analysis.

Conclusions:

  • Elevated UBE2C mRNA expression is a key driver of gastric cancer.
  • Targeting UBE2C or its regulatory pathways, such as microRNA-300, holds promise for gastric cancer therapy.
  • Further investigation into UBE2C-regulated genes may uncover novel therapeutic strategies.
Keywords:
Gastric cancerHuRUBE2CmRNA expressionmiR-300

More Related Videos

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

2.2K
miRNA Expression Analyses in Prostate Cancer Clinical Tissues11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

11.1K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.5K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.4K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
4.9K
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.5K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
7.4K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.9K