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Inflammatory Markers in Suction Blister Fluid: A Comparative Study Between Interstitial Fluid and Plasma.
Ulrika Sjöbom1,2, Karin Christenson3, Ann Hellström2
1Institute of Health and Care Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Frontiers in Immunology
|November 23, 2020
Summary
Interstitial fluid (ISF) and plasma show distinct protein profiles, but inflammatory markers are proportionally related. This suggests ISF is a promising biofluid for health monitoring and biomarker analysis in clinical settings.
Area of Science:
- Biomarker discovery
- Proteomics
- Clinical diagnostics
Background:
- Biomarker analysis aids disease detection, prediction, and health monitoring.
- Interstitial fluid (ISF) is an emerging matrix for biomarker studies.
- This research compares inflammatory markers in ISF (suction blister fluid - SBF) and plasma.
Purpose of the Study:
- To compare inflammatory marker levels between plasma and ISF (SBF).
- To evaluate the potential of ISF as a biofluid for health monitoring.
Main Methods:
- Collected plasma and SBF from 18 healthy individuals.
- Analyzed 92 inflammation-related protein biomarkers using Proximity Extension Assay (PEA).
- Compared protein profiles using statistical analyses.
Main Results:
- Quantified 70 proteins in both plasma and SBF.
- Observed distinct protein profiles with up to 40-fold differences.
- Found significant correlations for 25 proteins between plasma and SBF.
Conclusions:
- Skin ISF and plasma exhibit unique protein profiles.
- Many inflammatory markers show proportional relationships between ISF and plasma.
- ISF shows promise for biomarker monitoring in clinical studies and routine analysis.
