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Updated: Nov 29, 2025

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer
09:08

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

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SNORA72 Activates the Notch1/c-Myc Pathway to Promote Stemness Transformation of Ovarian Cancer Cells

Liwen Zhang1,2,3,4, Rong Ma1,2,3,4, Mengcong Gao1,2,3,4

  • 1Department of Pharmacology, School of Pharmacy, China Medical University, Shenyang, China.

Frontiers in Cell and Developmental Biology
|November 23, 2020
PubMed

Insights

Small nucleolar RNA SNORA72 promotes ovarian cancer stem cell properties, enhancing self-renewal and migration. This occurs via the Notch1/c-Myc pathway, offering a potential therapeutic target for ovarian cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Cancer stem cells (CSCs) drive tumor progression, metastasis, and recurrence.
  • Small nucleolar RNAs (snoRNAs) are implicated in various cancers, but their role in ovarian CSCs is largely unexplored.

Purpose of the Study:

  • To investigate the function of SNORA72 in regulating the stemness of ovarian cancer stem cells (OCSCs).
  • To elucidate the molecular mechanisms underlying SNORA72's effects on OCSCs.

Main Methods:

  • Serum-free culture of OVCAR-3 and CAOV-3 cells to induce OCSC characteristics.
  • Overexpression and silencing of SNORA72.
  • Assessment of self-renewal and migration abilities.
  • Analysis of stemness markers (Nanog, Oct4, CD133) and signaling pathway components (Notch1, c-Myc) via mRNA and protein expression.
  • Notch1 knockdown experiments.

Main Results:

  • SNORA72 was significantly upregulated in OCSCs.
  • SNORA72 overexpression enhanced OCSC self-renewal, migration, and stemness markers.
  • SNORA72 upregulated Notch1 and c-Myc expression.
  • SNORA72 silencing reversed these effects.
  • Notch1 knockdown counteracted the pro-stemness effects of SNORA72.

Conclusions:

  • SNORA72 activates ovarian cancer cell stemness through the Notch1/c-Myc pathway.
  • SNORA72 represents a novel therapeutic target for enhancing ovarian cancer treatment efficacy.
Keywords:
Notch1SNORA72c-Mycovarian cancer stem cells (OCSCs)stemness

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